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qPCR and RT-qPCR

Discover sensitive, dependable qPCR and RT-qPCR kits for probe- or dye-based real-time PCR and multiplex qPCR.

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qPCR and RT-qPCR

qPCR and RT-qPCR Kits for Probe-Based Detection

The GoTaq® Probe Systems provide ready-to-use 2X Master Mixes that simplify preparation of qPCR and RT-qPCR reactions for probe-based detection. Compatible with most real-time PCR instruments.

qPCR and RT-qPCR Kits for Dye-Based Detection

The GoTaq® qPCR and RT-qPCR Systems contain BRYT Green® dye, a proprietary dsDNA binding dye with brighter fluorescence and greater stability than SYBR® Green without the inhibition. Compatible with most real-time PCR instruments.

Primer-Based, Multiplex-Capable Real-Time PCR

Plexor® Systems offer real-time qPCR for sensitive and specific gene expression or SNP genotyping analysis, with melt curve analysis for specificity confirmation.

 

Műszaki specifikációk

Promega real-time PCR (qPCR) and RT-qPCR assays provide a choice of probe-based or dye-based qPCR methods. We also offer XpressAmp™ Reagents that are compatible for use with the GoTaq® qPCR and RT-qPCR Systems and enable direct PCR amplification from viral samples without having to perform RNA or DNA extraction.

Both probe and dye-based qPCR assay systems provide sensitive detection for reproducible and earlier quantification of low- and high-copy-number targets over a broad dynamic range, together with resistance to a wide range of PCR inhibitors. They provide sensitive detection on most real time PCR instruments and are amenable to both fast and standard cycling methods.

All real-time qPCR and RT-qPCR systems also include the convenience of room-temperature reaction set-up and easy-to-use master mix formulations.

 

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