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GenomeLab™ GeXP Genetic Analysis System

Complement your gene expression analysis with LOH studies. Confirm your SNP analysis results by sequencing. Combine MLPA genomic results with quantitative gene expression for the full story.

ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK!

Overview

The GenomeLab™ GeXP supports a variety of applications, including DNA sequencing and fragment analysis. Furthermore, this flexible system allows you to run more than one application on the same plate and up to 192 samples unattended.

Get the results you need all on one platform, using:

  • One Gel
  • One Capillary Array
  • One Software Package

A broad spectrum of applications:

  • Gene Expression
  • DNA Sequencing
  • Genotyping and SNP Analysis
  • STR Analysis
  • AFLP®*
  • MLPA**
  • LOH
  • MLVA


GenomeLab GeXP Genetic Analysis System Brochure

 

Genetic Analysis Features & Benefits

The GenomeLab™ GeXP Genetic Analysis System utilizes single or dual plates with the sample tracking technology option to provide an advanced, industry-leading genetic analysis solution. The result is a fully automated, high-resolution system that adapts well to daily workflow changes in sample type and complexity.

The GenomeLab™ GeXP has the ability to process and track samples in two 96-well plates. An array of eight capillaries takes full advantage of the 96-well plate format, enabling you to process over 192 samples, including thousands of genes, within 24 hours. This level of throughput reduces the cost and complexity associated with microarrays. Samples are automatically denaturated on-line prior to electrokinetic injection.

Long-Life Lasers

On-column, laser-induced fluorescence with auto capillary alignment ensures sensitive and reliable detection. Long life diode lasers are used to excite infrared dyes, providing higher sensitivity at a fraction of the cost of argon ion lasers.

Dual Plates

This system has the ability to process and track samples in two 96-well plates. An array of eight capillaries takes full advantage of the 96-well plate format, enabling over 192 samples, including thousands of genes, to be processed within 24 hours. A Single plate GeXP format is also available. Please ask your sales representative for more details.

Sample Tracking Made Simple

An integrated barcode reader ensures accurate sample tracking and reporting. It also lets you create GenomeLab™ GeXP sample setup with automated liquid handlers such as our Biomek® Series laboratory automation workstations.

Automatic Gel Loading

Because the gel is automatically loaded into the capillary array, you're assured of a consistent gel matrix for every run. The modular 20 ml gel cartridge are easily replaced.

Comprehensive Software Package

The GenomeLab™ software provides automated genotype calling and links to gene expression studies, association studies, pharmacogenomics and informatics. GenomeLab™ software also provides high quality sequence data analysis, ideal for genome sequence assembly, polymorphism studies, EST analysis and phylogenetic analysis.

Integrated Sample Handling

For sample preparation, the Biomek® series automated liquid handlers, as well as Allegra™ and Avanti® series centrifuges are fully compatible with the GenomeLab™ GeXP Genetic Analysis System.

WellRED Dyes

Beckman Coulter's WellRED dyes are uniquely designed for use with the GeXP Genetic Analysis System. The WellRED dyes emission spectra is in the near infrared region, providing greater sensitivity and significantly lower background than dyes that emit within the visible end of the spectrum. This results in minimal noise, which can interfere with peak detection and quantitation.

Identify Changes or Mutations in a Sequence

Our CEQuence Investigator automatically determines if the sequence orientation is forward or reverse, matches the orientation, forms consensus, aligns the consensus with a reference sequence, and provides a detailed report on the differences.

World-class Support at Your Fingertips

Wherever you are, our world-class customer service and support is dedicated to making sure your Beckman Coulter system functions at peak efficiency throughout its lifetime. Across the globe, a network of technical and application experts is available online, on site and by phone to help with all your system support needs.

 

WellRED Fluorescent Dyes

Long-Life Lasers

The WellRED fluorescent dyes from Beckman Coulter facilitate high sensitivity detection. Excitation of these dyes is achieved with a 650 nm and 750 nm diode laser. These diode lasers have a long life and are inexpensive to maintain. The emissions from these dyes are well separated and are in the infrared when there is less interference from biological materials. The WellRED dyes are available as dideoxy terminators, ddA, ddC, ddG, and ddT within the GenomeLab™ Dye Terminator Cycle Sequencing (DTCS) Kits, GenomeLab™ SNPStart Primer Extension Kit, or may be purchased as dye-labeled custom oligonucleotides from Proligo.

Additionally, the WellRED Dye-Labeled Phosphoramidites are easily coupled to the 5' end of oligonucleotides using commercial DNA synthesizers.

 

 

 

Műszaki specifikációk
  • 8 kapillárisos array bevont (coated) kapilláris az EOF (electro-osmotic flow) csökkentésére
  • Lézer indukálta fluoreszcenciás detektálás
  • Fényforrás: 2 db far-red dióda lézer (hullámhossz: 650 és 750 nm) alkalmazása az alacsony zaj elérése érdekében
  • 4-féle jelölés egyidejű leolvasása: lézerenként 2 festék kerül gerjesztésre, így könnyebb a spektrum átfedésekből adódó nehézségek (color compensation) kompenzációja – szemben az 1 lézer > 4 festékkel alkalmazottakkal.
  • Mivel a dióda lézer intenzitása több ezer munkaórán túl is szinte változatlan, a matrix kompenzációt csak egyszer kell elvégezni.
  • 96 minta (8 x 12)  beavatkozásmentes, automatizált feldolgozása
  • Automatikus minta denaturáció (a készülék maga végzi), minta- és gélbetöltés
  • Egyféle, lineáris poliakrilamid alapú – nem mérgező - univerzális gél  szekvenáláshoz és fragmens analízis módszerekhez, valamint multiplex génexpressziós vizsgálatokhoz egyaránt
  • A futtatásra előkészített minták formamid tartalmú oldatban (sample loading solution) inert olajjal lefedve -20 fokon akár 1 hónapig is tárolhatók a microplate welljeiben.
  • Korlátlan számú szoftverlicenc
  • A készülék alkalmas akár 30 gén költséghatékony multiplex génexpressziós vizsgálatához alacsony (20 ng totál RNS/minta) mintaigénnyel
  • Egyféle kapilláris rövid és hosszú szakaszok szekvenálására, valamint fragmensanalízisre és génexpressziós vizsgálatra egyaránt
  • Egy plate-n belül a 8-kapillárisnak megfelelően 12, egymástól eltérő applikáció futtatása.
  • Különálló szoftver multiplex esszé tervezéséhez és analízishez
  • Nyílt rendszert biztosít más multiplex génexpressziós kémiához is.
  • Támogatja a következő génexpressziós panelek analízisét: humán és patkány referencia gének, humán mellrák, humán metasztázis
  • Szekvenálás
  • 700 bp leolvasása 75 percen belül
  • 96 minta szekvenálása 900 bp-ig 24 óra alatt
  • 500 bp leolvasása 45 percen belül
  • 96 minta szekvenálása 500 bp-ig 9 óra alatt
  • 98%-nál jobb leolvasási pontosság pUC18 templátnál
  • Hosszabb szekvenálások akár 900 bp-ig
  • Kimagasló eredmények problémás templátok esetén (GC-gazdag szakaszok, mikroszatelliták)
  • Teljes Phred/Phrap kompatibilitás
  • Adatkimenet SCF, SEQ, FASTA, PHRAP és ASCII formátumokban
  • Fragmensanalízis
  • Felbontóképesség: 1 bázis 60-tól 400 bp-ig
  • Felbontóképesség: 2 bázis 600 bp-ig
  • 8 minta feldolgozása 45-55 percenként (400 és 600 bp között)
  • 96 minta feldolgozása 9-12 órán belül a betöltéstől az adatexportig
  • Multiplex reakciók akár 20 lokuszig, well-enként 4 festék használatával (Cy5 kompatibilitás)
  • Automatikus allél meghatározás
  • Mikroszatellit amplifikációs termékek méretének pontos meghatározása (S.D. < 0.27 bázis)
  • SNP értékelés, STR, MLPA, RFLP, tFLP, LOH, AFLP applikációk
Tartozékok, kiszerelések

Genetic Analysis Software

Perform gene expression, STR, SNP, AFLP and other distinct DNA fragment sizing and identification applications using the GenomeLab™ GeXP Genetic Analysis System. Whether you're mapping inborn errors of metabolism, genotyping an individual, or quantifying microsatellite instability, the accurate and timely assignment of alleles can dramatically impact your lab's productivity. The GenomeLab™ GeXP Series Software Suite provides high- precision DNA sizing and sophisticated software algorithms with these processes in mind. The GenomeLab™ Software Suite also provides high quality sequence data analysis, ideal for genome sequence assembly, polymorphism studies, EST analysis and phylogenetic analysis.

 

Alleles may be identified by size or a combination of size and color allowing both STR and SNPs to be identified from the same simplified setup.

 

All parameters associated with the results are at your fingertips, allowing data to be reviewed with ease. Electropherograms may be further stacked and overlaid or stacked to facilitate data comparison.

 

Accurate & Timely Assignment of Alleles

You can identify alleles even in the presence of enzyme stutter and the plus Adenosine artifact. The GenomeLab™ software automatically assesses quality and highlights results that need further review, allowing you to rapidly assess and interpret your data.

 

Data Review & Organization

The GeXP Series delivers precise DNA sizing, accurate allele assignment and automated data review. It has a sophisticated set of tools for managing and reviewing data, with sorting and filtering tools that operate on a set of over 100 independent properties. Individual results can be mapped to your personal preference to automatically review the data.

 

Data Output

The GenomeLab™ Software allows for several formats of reporting/exporting analyzed data.

The Genotype Summary report can be exported for linkage/association studies.

Analyzed data can also be presented in semi-custom reports based on Microsoft Word templates or exported in multiple formats (*.csv, *.txt, *.crv, *.pre,*.gsr) to be used in third party data analysis applications.

 

Secondary Analyses

 

The GenomeLab™ Software provides integrated Binning processes to facilitate Locus tag generation.

 

Efficient tools for quantifying DNA fragments using peak area, peak height, and ratios of standards with references, as well as ratios of alleles for loss of heterozygosity detection are also provided.

 
Gene Expression Anaylsis

The GenomeLab™ GeXP has a sophisticated set of software tools that will guide you all the way from designing primers to data management of high-throughput gene expression studies.

eXpress Profiler

GenomeLab™ eXpress Profiler takes the uncertainty out of assay design by providing fast, automated primer design, multiplex development and calculation of relative gene expression values.

eXpress Analysis

With the eXpress Analysis module, fragment data is easily identified and associated with the design file using the software's QC functionality. This analysis can be imported into GeXP Quant Tool, third-party analysis software or LIMS

Normalize to Multiple Genes

The power of multiplexing allows for the inclusion of several reference (housekeeping) genes in the same reaction. The GenomeLab™ GeXP makes it possible to normalize gene expression data to multiple reference genes at once, giving you the unprecedented flexibility to choose the best reference gene or genes in your assay system.

 
 
DNA Sequence Analysis - Automated Base Calling

The GenomeLab™ sequence analysis software automatically calls each base and assigns a confidence value to the call to allow you to immediately assess the quality of the sequence data. Sequence analysis includes signal processing, base calling and the determination of confidence values for each called base. No calibrations are required to compensate for spectral overlap or mobility variations between the dyes. A two pass base-caller first identifies significant peaks then employs a directed graph technique** to refine the called base sequence. Finally, the error-probabilities of called bases are estimated and quality values are assigned. Automated heterozygote detection can also be selected allowing the user to identify heterozygous base positions in genomic DNA.

 
 
Multiple Analysis Capabilities
Automatic base calling and report generation after each row of eight samples
Editable parameter set for base calling algorithm
Audio playback of selected DNA segments
Call scores and quality values generated for each sequence for base call assurance with quality score based trimming
Exporting capability for third-party data analysis (.scf, FASTA, PHRED, SEQ, Tab-Delimited)
 

 

 
Applikációk

Finally, a multiplexed, quantitative gene expression solution that meets your needs and is cost effective!

Imagine being able to multiplex up to 30 genes in one reaction, quantitatively and reproducibly, using only a small amount of your precious RNA sample. Imagine no longer! You can now achieve this with our highly multiplexed reverse transcription PCR* approach (XP-PCR*) to quickly and efficiently look at the expression of multiplexed gene sets with greater sensitivity, speed and cost effectiveness compared to singlet and multiplex based-q PCR* approaches.

 

The GenomeLab™ GeXP Genetic Analysis System can accurately detect as low as 0.5-fold changes in gene expression, providing more precise information than ever before. Powered with our unique universal priming strategy, XP-PCR* allows multiple reference genes, genes of interest and an internal control to be unbiasedly analyzed in a single well. This enables the most accurate normalization and leads to reliable detection of very small changes in gene expression.

 

The GenomeLab™ GeXP Genetic Analysis System is ideal for multiplex panel validation and pathway studies. Focus areas include stem cell pluripotency studies1, cancer research utilizing FFPE samples, multiplex microbial detection for respiratory virus identification2 andfood & beverage bacteria and yeast characterization. The GeXP also supports researchers who have completed their initial discovery work from the literature or large-scale screening technologies. The GeXP system is used for multiplexed, quantitative gene expression analysis as well as a multipurpose genetic analysis platform.

 

Gene Expression Solutions Benefits

High-throughput quantitative gene expression

With the capacity to analyze up to 30 genes per reaction, the scalable GenomeLab™ GeXP enables the examination of up to 5,760 data points unattended in 24 hours.

Cost-effective and time-saving gene expression

The GenomeLab™ GeXP System can lower your PCR* expenses and improve efficiency. Here's how: the multiplex power built into the GeXP enables you to analyze up to 30 genes per sample at a dramatically reduced cost-per-gene expression result with considerable time savings.

High accuracy and reproducibility you can trust

With unrivaled linearity (R2 > 0.99 for most genes), the GeXP delivers precise gene expression profiling that can detect gene expression changes down to 0.5 fold. A very high signal-to-noise ratio increases sensitivity and reproducibility across samples for more accurate and informative results.

Accurate representation of your targeted genes

Don't assume the amplified products in your PCR reaction are purely the intended target. Alternative and psuedogene transcripts can be amplified by homologous primer sequences. Separation of PCR products by size using capillary electrophoresis allows for identification and removal of unintended products, both specific and non-specific, from quantitation. This results in highly accurate and precise quantitation of the specific, intended gene target. This advantage is unique to the multiplexed, reverse transcription PCR approach and capillary electrophoresis technology offered by the GenomeLab™ GeXP gene expression profiling (XP-PCR) process. This translates to the most accurate quantitation in a multiplex environment.

Low sample requirement

Our multiplexing capability, coupled with capillary electrophoresis readout, can be efficiently used to look at focused sets of genes using as little as 5-50 ng of total RNA.

Comprehensive software tools

The GenomeLab™ GeXP has a sophisticated set of software tools that will guide you all the way from designing primers to data management of high-throughput gene expression studies.


Multiplexed Gene Expression Delivers High Sensitivity, Accuracy and Precision

Linearity and Precision

The GenomeLab™ GeXP produces gene expression data with superb linearity. A highly linear correlation between the amount of RNA and gene expression level is generated for each gene in a multiplex with an average correlation coefficient (R2) well above 0.99.

Accuracy

The GenomeLab™ GeXP is sensitive enough to precisely detect even small changes in gene expression. The 0.5-fold increases in RNA concentration are consistently and accurately quantified by the GeXP for all genes in a multiplexed assay.

Human Breast Cancer Electropherogram

Simultaneously analyze 24 functional genes associated with breast tumor progress and three reference (housekeeping) genes.


Stem Cell Analysis Solutions

Complete Stem Cell Characterization

Addressing the workflow challenges associated with stem cell screening, the GenomeLab™ GeXP Genetic Analysis System** provides a complete solution in a single platform. Integrating the GeXP Start Kit and Pluripotency panel for gene characterization with the GeXP STR Kit** for contamination detection, it accelerates screening for pluripotent stem cell lines. And together with support from Beckman Coulter's team of dedicated technical experts, it makes research and quality control processes more effective and efficient — saving you time and money.


Product Features

GenomeLab™ GeXP Gene Expression Profiling and Fragment Analysis
  • Enhance efficiency with an integrated single-platform solution utilizing the reliability of capillary electrophoresis one array, one gel and one software
  • Examine over 5,700 data points unattended per day with highly accurate relative quantitation of 2-30 genes per reaction
  • Overcome RNA restraints with a requirement of 5 to 50 ng total per reaction
  • Ideal for biomarker signature validation and subsequent screening as well as unique and challenging samples like iPSC colonies, single cell solution and simultaneous detection of multiple transcript variants
GeXP Start Kit with Pluripotency Panel
  • Monitor and characterize cell lines at a molecular level with more sensitivity and specificity
  • Compare delivery methods for reprogramming factors
  • Characterize a biomarker signature for each step of somatic cell reprogramming and differentiation of specific lineages
  • Readily identify and assess the pluripotent capacity of individual colonies from non induced lines
  • Monitor pluripotency of stem cell lines
  • Eliminate individual labeling of primers with versatile universal amplification kits
GeXP Human STR Primer Kit
  • Increase confidence in data source by tracking cell lines from the differentiated to pluripotent state and back to differentiated - Monitor and quantify contaminants
  • Utilize 12 loci to identify and track human cell lines from differentiation to iPSC to re-differentiation
Next Generation Pluripotency Biomarker Screening
  • Expedite the process and save time with the only system that offers multiplexing with a universal PCR* primer strategy
  • Save on primer synthesis with the dye-labeled primer incorporated into the GeXP Start Kit
  • Analyze up to 30 genes per reaction with relative quantitation with unrivaled sensitivity and reproducibility
World-Class Customer Service and Support
  • Get technical and applications support on-line, on-site and by phone
  • Receive on-site installation and training for instrument and methods
  • Count on complete post-sales hardware, software and method support
Videók, ismertetők

 

 

Literature
Brochures / Data Sheets
Automated Multiplexed Gene Expression Profiling
GenomeLab GeXP Genetic Analysis System
Authenticate Your Human Cell Lines
CEQ 8000 AFLP Analysis
CEQ 8000 STR Analysis
GenomeLab GeXP Series SNP Analysis
GenomeLab DTCS Quick Start Kit for Dye Terminator Cycle Sequencing
GenomeLab Human STR Primer Set
Sequencing Template Conversion
The eXpress Profiling Process for Gene Expression Analysis
WellRED Dye-Labelled Phosphoramidites

Posters
A Multiplexed Capillary Electrophoresis Method to Screen Beer-Spoilage Bacteria and Wild Yeast
A Novel Strategy for the Simultaneous Detection of Alternatively Spliced Transcripts: A Study of MEF2C Exon Covariation
High-Throughput, Quantitative Multiplex Gene Expression Assay Accelerates The Validation Of Breast Cancer Biomarkers
Rapid and Accurate Identification of Human Induced Pluripotent Stem Cells with a Novel Multiplex Gene Expression Assay
Validation of a 16-Gene Expression Signature in Non-Small-Cell Lung Cancers from FFPE Samples

Technical Application Information
A Rapid and Efficient Method for the Post-Reaction Cleanup of Labeled Dye Terminator Sequencing Products Using the Avanti J-E Centrifuge with the AllSpin JS-5.3 Rotor
AFLP Protocol For Plant Science on CEQ Series Genetic Analysis System
Allele Size and Plus A in CEQ Fragment Analysis Software
Analysis of 26 Weber Panel Simple Tandem Repeat Polymorphisms Using the CEQ 2000 Fragment Analysis System
Automated Binning Process for the Generation of Locus Tags
Automating Heterozygote Detection Using the Human p53 Gene as a Model System
Bacterial Artificial Chromosome (BAC) End Sequencing Analysis
Call Scores and Quality Values: Two Measures of Quality Produced by the CEQ Genetic Analysis Systems
Highly Precise DNA Sizing on the CEQ Genetic Analysis System
Improved Sequencing of Plasmids on the CEQ 2000 by a Simple Template Preheating Procedure
Method for Long Sequencing Read Lengths on the CEQ Genetic Analysis System
Monitoring Multiple Pluripotency Biomarkers After Delivery of Reprogramming Factors to Human Somatic Cells
Multiplex Mutation Genotyping for Human Diseases: Breast Cancer and Familial Mediterranean Fever
Multiplex SNP Analysis: Screening Factor V R506Q (Leiden) Mutations
Multiplexed, Quantitative Gene Expression Analysis for Lettuce Seed Germination on GenomeLabT GeXP Genetic Analysis System
Post Sequencing Reaction Purification in 96 Well Gel Filtration Column Plates
Quantitative Multiplexed Gene Expression: An Analysis of Sensitivity, Accuracy and Precision
Sequencing of Difficult DNA Templates with the GenomeLab Methods Development Kit
SNPs. Mutations and DNA Sequence Variation Analysis Using the GenomeLab SNPStart Kit
Strategies for Automating the Review of Data
The Use of AFLP Techniques for DNA Fingerprinting in Plants



      
Technical Documents
GenomeLab DNA Size Standard 80 Kit (608398)
GenomeLab Dye Terminator Cycle Sequencing with Quick Start Kit (608118)
GenomeLab Fragment Analysis Protocol (608113)
GenomeLab Genetic Analysis System User's Guide (A29142)
GenomeLab GeXP Chemistry Protocol for Gene Expression (A29143)
GenomeLab GeXP Genetic Analysis System Pre-Installation Guide (A32350)
GenomeLab GeXP Human Breast CancerPlex Kit (A32698)
GenomeLab GeXP Human MetastasisPlex Kit (A32714)
GenomeLab GeXP Human ReferencePlex Kit (A21780)
GenomeLab GeXP Rat MultitoxPlex Kit (A21782)
GenomeLab GeXP Reference Workflow for Gene Expression (A54011)
GenomeLab GeXP Start Kit (A85019)
GenomeLab GeXP Troubleshooting Guide for Gene Expression (A53995)
GenomeLab Human STR Primer Set (A20103)
GenomeLab Methods Development Kit Dye Terminator Cycle Sequencing Protocol (608019)
GenomeLab Sequencing Chemistry Protocol (390003)
GenomeLab SNP Primer Extension Kit (390315)
GenomeLab SNPStart Primer Extension Kit (A23206)
GenomeLab SNPStart Primer Extension Kit with CD (A26470)
Labeling and Purification of Oligonucleotides with the Beckman Coulter, Inc. WellRED Dye-labeled Phosphoramidites (390012)
Software
eXpress Profiler Patch
GenomeLab Algorithm Update
GenomeLab GeXP Plex TDF Download
GenomeLab GeXP Quant Tool
GenomeLab Human STR Primer Kit Locus Tags



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