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Promega

Riporter rendszerek és transzfekció

Directly visualize and quantify apoptosis in any cell line or transgenic mouse expressing firefly luciferase with a soluble, nontoxic substrate.
Detect reporter gene activity with luciferase-based assays just right for your application. Use classic Dual-Luciferase® Assays or state-of-the-art NanoDLR™ and Nano-Glo® Luciferase Assays with NanoLuc® Luciferase for unrivaled sensitivity and versatility.
Rapidly develop sensitive reporter assays using robust reporter vectors, luciferase reporter genes and cell lines integrated with luciferase reporter technology. Choose a reagent to deliver DNA into your cell line of interest with high efficiency and low toxicity.

ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK!

In vivo Imaging

Soluble, Non-Toxic in vivo Imaging: VivoGlo™ Caspase 3/7 Assay

Directly visualize and quantify apoptosis with any cell line or transgenic mouse expressing firefly luciferase.

EnduRen™ Live Cell Substrate

Quantitate Renilla luciferase in live cells with high signal-to-background ratios and extended signal kinetics. Ideal for use with kinetic reporter gene analysis, BRET and ddRNAi.

Reporter Assays

Nano-Glo® Luciferase Assay System

This one-step assay will detect NanoLuc® Luciferase, an all new, state-of-the-art technology offering unrivaled sensitivity and versatility in a very small reporter.

ONE-Glo™ EX Luciferase Assay System

A simple, sensitive "add-mix-read" firefly luciferase reporter assay. Provides long-lived luminescence ideal for batch processing multiple 96-, 384- or 1,536-well plates.

ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay

The ONE-Glo™ + Tox Assay combines luciferase assay chemistry with a cell viability marker to better understand reporter gene expression in the context of cell health.

Bright-Glo™ Luciferase Assay System

This assay offers the brightest, most sensitive option for mix-and-read detection of firefly luciferase. It is compatible with high-throughput formats, especially when used in a continuous process format.

Dual-Glo® Luciferase Assay System

This homogeneous reagent system offers fast and simple quantitation of cotransfected  firefly and Renilla luciferase activities.

Dual-Luciferase® Reporter Assay System

This non-homogeneous reagent system is the most sensitive method for quantifying cotransfected firefly and Renillaluciferases.

Reporter Vectors and Cell Lines

NanoLuc® Luciferase Reporters

State-of-the-art reporter technology offering unrivaled sensitivity and versatility in a very small luciferase. Find the pNL reporter that is right for your application.

Coincidence Reporter Vectors

Express firefly and NanoLuc® luciferases from the same transcript. Generate two unfused luciferase enzymes with distinct compound interaction profiles, allowing convenient identification of true and false hits in HTS compound screening.

pmirGLO Dual-Luciferase miRNA Target Expression Vector

Quantitatively measure microRNA activity with a luciferase reporter. The firefly luciferase (luc2) vector is the primary reporter to monitor mRNA regulation and Renilla luciferase (hRluc-neo) acts as a control reporter for normalization and selection.

pGL4 Luciferase Reporter Vectors

These next generation, luciferase reporter vectors offer more efficient expression of both firefly and Renilla luciferases in mammalian cells. 

 

Transfection Reagents

ViaFect™ Transfection Reagent

Generate more relevant data with efficient transfection of your optimal cell line without compromising cell viability.

FuGENE® 6 Transfection Reagent

FuGENE® 6 offers proven performance cited in thousands of scientific papers and used in hundreds of cell lines. The novel non-liposomal formulation offers high efficiency transfection of DNA with minimal toxicity.

FuGENE® HD Transfection Reagent

Transfect DNA into a wide variety of cell lines with high efficiency and low toxicity! There is no need to remove serum or culture medium after introducing the reagent:DNA complex.

TransFast™ Transfection Reagent

Spend less time optimizing transfections especially for primary cell cultures requiring continuous exposure to serum.

 
 
 

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