JM Modern HikaShop - шаблон joomla Видео
In
(06-1) 619-8321
Promega

NanoBiT™ Complementation Assay for Protein Interactions

For three out of the last four years, Promega has been honored to have one of its key technologies named a Top 10 Innovation by The Scientist.
In 2015, the innovative NanoBiT™ Assay (NanoLuc® Binary Technology) received the recognition.

ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK!

NanoBiT™ is a structural complementation reporter based on NanoLuc® Luciferase, a small, bright luciferase derived from the deep sea shrimp Oplophorus gracilirostris.

 

Using plasmids that encode the NanoBiT complementation reporter, you can make fusion proteins to “report” on protein interactions that you are studying. One of the target proteins is fused to the 18kDa subunit; the other to the 11 amino acid subunit. The NanoBiT™ subunits are stable, exhibiting low self-affinity, but produce an ultra-bright signal upon association. So, if your target proteins interact, the two subunits are brought close enough to each other to associate and produce a luminescent signal. The strong signal and low background associated with a luminescent system, and the small size of the complementation reporter, all help the NanoBiT™ assay overcome the limitations associated with traditional methods for studying protein interactions.

 

NanoBit

 

The small size reduces the chances of steric interference with protein interactions. The ultra bright signal, means that even interactions among proteins present in very low amounts can be detected and quantified–without over-expressing large quantities of non-native fusion proteins and potentially disrupting the normal cellular environment. And the NanoBiT™ assay can be performed in real time, in live cells.

NanoBIt forskolin

NanoBit ordering 

More details

blue down arrowForgalmazott termékeink gyártói - keressen gyártó szerint a logóra kattintva