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Fluxion Biosciences

IONFLUX MERCURY ULTRA

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Mercury Ultra takes the leading IonFlux APC to the next level in throughput and flexibility

IonFlux Mercury Ultra is the latest iteration of the industry-leading IonFlux platform, with enhancements in every aspect of the technology. With 256 independent amplifiers capable of non-stop recordings, and an uninterrupted sweep length of up to 300 seconds, IonFlux Mercury Ultra has multiplied the IonFlux platform’s high throughput capabilities. IonFlux Mercury Ultra extends the IonFlux system’s industry-leading capabilities in compound delivery, rapid solution exchange, and assay flexibility. Now, the most complicated ligand-gated and demanding voltage-gated assays can run in parallel, decreasing experiment time and accelerating completion of projects.

ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK!

IonFlux Mercury Ultra delivers these unique benefits:

  • Continuous flow of solutions and compounds with integrated wash steps for maximum assay stability and fidelity

  • 256 independent recordings with 128 parallel experiments provide a fast execution of the most demanding assays

  • Simultaneous ligand- and voltage-gated assays

  • Simultaneous single cell giga-ohm seal recordings and ensemble recordings (20 cells) without artificial averaging

  • Integrated temperature control

  • Synchronous and asynchronous assay operation

  • Ultra fast execution of experiments (10 minutes for a 6 point dose response) allows for more than 10,000 data points per hour and more than 50,000 data points per day

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Műszaki specifikációk

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Applikációk

 

FEATURED APPLICATIONS

Due to its unique liquid exchange system, IonFlux is the ideal platform for ligand binding, allosteric modulation, multiple agonists, and voltage- and temperature-sensitive assays. It is used in laboratories and research centers worldwide for various voltage-gated and ligand-gated ion channel assays.

 
 
VIROPORINS

and therapeutic possibilities for COVID-19

 
 
nACh RECEPTORS

and neuronal transmission

 
 
GABAa RECEPTORS

and neuronal excitability

 
 
POTASSIUM CHANNELS

and stability in safety pharmacology

 
 
SODIUM CHANNELS

and pain & toxins research

 

Tartozékok, kiszerelések

 

Unique in-plate liquid handling, patching and recording

IonFlux uses a unique plate design that allows for all fluid activity to be conducted within the recording plate proper. Using flexible and user controlled pneumatic system, IonFlux provides high speed liquid exchange, continuous solution flow, and drug delivery without the use of an external liquid handler. The in-plate movement of solutions cancels typical ion channel assays problems such as stacking related drug cross contamination. The continuous flow system alleviates assays with slow acting or sticky compounds.

 

DESIGN

IonFlux Plates are microfluidic devices used exclusively in the IonFlux System for automated patch clamp assays. They are integrated into SBS-standard well plate formats for convenience and compatibility with automated liquid handling instrumentation. Multiple plate formats are available which correspond to the IonFlux system configuration. Each well on the plate has a dedicated function as either a compound well, cell trapping well, cell inlet or cell outlet well.

Plates are divided into experimental patterns. Each pattern contains specified wells:

  • Two Independent trapping wells: These wells would contain intracellular solutions

  • Cell inlet well: This well would contain the prepared cells in extracellular solution

  • Cell outlet well: Acts as a waste well for the pattern

  • Eight independent compound wells: These wells would contain any combination of different compound solutions or dose response concentration gradient of the same compound. IonFlux software provides full control over these compound wells release and timing.

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FORMATS

IonFlux plates come in two different standard designs, 96 well plates for use with all IonFlux system having 16 amplifiers and 384 well plates for use with all IonFlux systems with 64 amplifiers or more.

96 well plate - used exclusively with IonFlux Classic 16 & IonFlux Mercury 16

384 well plate - used exclusively with IonFlux Mercury HT and IonFlux Mercury Ultra

 

RECORDINGS

IonFlux plates come in two different cell trapping and recording designs:

  • Single Cells: Using whole cell patch clamp technique and achieving giga-ohm seal recordings, IonFlux single cell plates are perfect for specialized assays requiring complete voltage/current control of an independent cell, mimicking manual patch clamp conditions while providing automated systems flexibility.

  • Ensemble cells: Just like single cell plates, ensemble plates also use whole cell patch clamp techniques but now with 20 cells in parallels setting a standard for consistency and throughput All IonFlux instruments can use either plate design with no hardware modifications.

 

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