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Beckman Coulter

MoFlo® Astrios™

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Leveraging the historically stable fluidic design of the original MoFlo and the electronic processing of the MoFlo XDP, the MoFlo Astrios expands sorting capabilities with a full palette of laser options. Seven pinholes spatially separate each laser, providing the flexibility and expandability known to MoFlo sorting.

ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK!

The MoFlo Astrios is the new standard for cell sorting with functionality, speed, and improved sensitivity. It is designed for researchers who desire high productivity with more analytical capability and with biosafety solutions. The ergonomic design of Astrios makes it faster and easier to operate. All components requiring operator control are within easy reach.

Beyond the optic expansion, novel software components enhance the instruments capabilities. The Touch Screen Control Panel facilitates daily set up with its operation designed around core laboratory workflow. Auto Start Up enables the user to pre-define when the lasers and fluidics will start up, saving valuable time each morning. Creating flat-top beam profiles, a multi-fiber Beam Shaping Optic (BSO) shortens alignment time and improves optic stability. Two stages control the alignment for all 7 lasers, simplifying the sorter preparation. 

Auto QC allows system performance to be tracked and trended across 32 available parameters. IntelliSort II is a fully bead-less drop delay determination and monitoring system. Camera images and proprietary algorithms enable the optimal drop delay to be determined without the use of fluorescent particles in the sample line. An updated version of Summit Software containing 6-way sorting, plate sorting up to 1536-well, and Index sorting logic is standard. The Quick Release nozzle design enables removal or swapping of the nozzle body and tip through the use of single lever.

Designed from the start with biosafety considerations, MoFlo Astrios has comprehensive aerosol evacuation design that filters particles from all chambers where aerosols have the potential of being generated. A fully sealed sort chamber housing the redesigned R-Theta CyClone arm facilitates the aerosol evacuation capabilities which are tested to the current ISAC guidelines. Additionally, the MoFlo Astrios has a custom Biosafety Level II cabinet option. The BSL II cabinet is tested to NSF-49 International standards with the MoFlo Astrios inside.

For Research Use Only. Not for use in diagnostic procedures.

Műszaki specifikációk

MoFlo Astrios Optics:

  • 7 laser / 7 pinhole configurable system; 355nm, 405nm, 488nm, 532nm, 561nm, 592nm, 642nm
  • Single multi-fiber Beam Shaping Optic (BSO) for fiber coupled lasers
  • Flat-Top beam profile on fiber coupled lasers increases beam stability and power, alignment ease
  • 7 lasers aligned through two alignment stages for quick sorter set up

MoFlo Astrios Software:

  • IntelliSort™ II - The only sorter with a fully bead-less drop delay determination and monitoring
  • Touch Screen Control Panel for instrument set up and sort monitoring (intuitive operation and cross-training)
  • Auto Start Up enables lasers to automatically turn on and fluidics to pressurize
  • Single button Auto QC for tracking and trending with enhanced troubleshooting guidance

MoFlo Astrios Performance:

  • 6-way sorting with a variety of collection devices (temperature control for all sort outputs)
  • Mixed Mode sorting: each sort stream is capable of having its own sort mode (Enrich, Purify, Single) programmed. Soft aborts and waste can be collected for reanalysis or resorting.
  • Index sorting with intuitive mapping of events
  • Reproducibly sort up to 1536-well plates (due to historically stable fluidics)
  • Only sorter capable of >70,000 sort decisions/second validated performance

MoFlo Astrios Electronics and Software:

  • Electronic capability to simultaneously detect 32 parameters
  • 32 bit processing and 100 MHz sampling speeds
  • Zero electronic dead time equates to no hard aborts
  • >100,000 acquisition rate/second validated performance
  • 5-decade dynamic range PMTs
  • FSC and SSC off each wavelength greater than or equal to 405nm
  • Height, Area, Width, Log, Log Area collected simultaneously for all parameters
  • Describes regions mathematically at the full resolution of the parameter
  • 1 billion events .fcs files with no parameter limit

MoFlo Astrios Biosafety

Protecting you - Protecting your sample
Designed from the start with biosafety considerations, MoFlo Astrios has comprehensive aerosol evacuation design that filters particles from all chambers where aerosols have the potential of being generated. A fully sealed sort chamber housing the redesigned R-Theta CyClone arm facilitates the aerosol evacuation capabilities which are tested to the current ISAC guidelines. Additionally, the MoFlo Astrios has a custom Biosafety Level II cabinet option. The BSL II cabinet is tested to NSF-49 International standards with the MoFlo Astrios inside.

  • Fully tested to NSF-49 specifications custom Biosafety Level II cabinet option
  • Quick Release nozzle design for easy access to nozzle tip
  • Sort chamber free of hard edges designed for aerosol containment and cleanability
  • R-Theta CyClone® arm with wiper (seals back of sort chamber)
  • Aerosol Evacuation System (AES) pulling particulates from three (3) chambers with verified performance to meet the ISAC guidelines

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Enhanced Forward Scatter (eFSC)

With the MoFlo Astrios EQ, you never have to be limited by your instrumentation. Its patent pending dual enhanced forward scatter (eFSC) technology enables you to see and sort particles you might otherwise miss. Measure crucial particle characteristics over more than three orders of magnitude, from as small as 200 nm to 30 µm diameter, all at the same time. You can also trust MoFlo’s proven fluidics to be gentle with your biological material.

  • 7 lasers, 2 alignment stages
  • Resolve particles (beads and cells) down to 0.2 µm
  • Sort rare event populations with 99% purity
  • 6-way mixed mode sorting gives you flexibility in experiment design
  • Forward scatter detection support for 6 laser paths
  • Optional side scatter off any laser (excluding UV)
  • Minimum triggering threshold reduced to 0.001% to allow more resolution.
  • Seven mask designs for differing applications
  • ABS ND filter set. 0.3, 0.6, 1.0, 1.3, 2.0
  • Fluidic and air filtration enhancements:
    • Sheath filter 0.04 µm particles
    • Air filter 0.01µm particles
    • Unequalled resolving FSC & FL power through adjustable 9-decade dynamic range delivering greater certainty when resolving background from dim staining populations and/or broad dynamic range applications

Excitation Optics

Laser Palette:

Wavelength Power out of Laser Characterization
355nm 100 mW Elliptical shape
405nm 55 mW Flat-Top shape
488nm 200mW Flat-Top shape
532nm 150 mW Flat-Top shape
561nm 200 mW Flat-Top shape
592nm 200 mW Flat-Top shape
642nm 100 mW Flat-Top shape

Number of laser interrogation points: 7 discrete spots

Number of Pinholes: 7 Pinholes

Standard Filter Sets optimized per laser wavelength:
355nm – PI, Hoechst (red and blue), DAPI
405nm – SSC, Cascade Blue*, Pacific Blue*, Cascade Yellow*
488nm – SSC, FITC, PE, ECD (PE-Texas Red*), PE-Cy5/PerCP, PE-Cy5.5, PE-Cy7
532nm – SSC, PE, PE-Alexa* 610, PE-Alexa 647, PE-Cy5.5, PE-Alexa 700
561nm – SSC, mCherry, DSRed, mPlum, HCRed
592nm – SSC, Texas Red, APC, APC-Cy7, Alexa 647, Alexa 700, Alexa 750
642nm – SSC, APC, APC-Cy7, Alexa 647, Alexa 700, Alexa 750

Bringing new data within reach

Emission Optics

Resolution:
Resolves minimum 0.2μm diameter particles in scatter from background.
Fluorescence Sensitivity:
Fluorescence sensitivity of < 125 MESF for FITC and < 110 MESF for PE using
Spherotech 8-peak beads using 488nm laser
Optical Parameters:
Up to 44 including 2 FSC.
Fluorescence half height CV precision:
FITC <2.4%, PE <2.4%, PE-TR <2.6%, PE-Cy5 <3.2%,
PE-Cy7 <4.3%, APC <2.3%,
APC-Cy7 <2.8%

(1 x 106 SPHERO™ Ultra Rainbow beads at sample flow rate of 6 μL/min, 100-120 EPS)

IntelliSort II Beadless Drop Delay

Minimize your risk of sample contamination and maintain integrity of sorted samples with uninterrupted aseptic sorting.

  • No fluorescent beads or microscope required
  • Camera images and proprietary algorithms enable operator to determine optimal drop delay without the use of fluorescent particles in the sample line
  • Eliminates requirement to remove sterile samples during drop-delay recalculation due to nozzle blockages, thus ensuring uninterrupted sterility
  • Maintain drop delay functionality with 50µm and 150µm nozzle tips
  • New Auto-Quality Control parameters: sheath pressure, sample pressure, average event rate, start and end times, nozzle size, differential between measured parameters and pass/fail thresholds, and the ability to exclude QC data
  • New laser-delay parameters: date of last laser delay calculation, trigger channel, sheath pressure, nozzle size, laser wavelengths that are powered, calculated laser delays displayed

Summit v6.2 Software

Enjoy faster, easier instrument set-up and leading edge features and functionality.

  • Support for multiple forward scatter parameters in Summit and FCS file format
  • 2 additional PMT channels for forward scatter collection
  • Minimum allowable PMT voltage changed from 400 volts to 100 volts to enhance useable dynamic range.
  • Enhanced functionality and support for user-defined filters and fluorochromes.
  • Enhanced automated quality control functionality
Videók, ismertetők

Astrios EQ brochure

 


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  The Evolution Continues
Researchers rely on Beckman Coulter's historic cell sorter technology. Now the next generation is here. The MoFlo Astrios is the new standard for cell sorting with functionality, speed, and improved sensitivity. It is designed for researchers who desire high productivity with more analytical capability and with biosafety solutions. 
Applikációk

Application Flexibility

The MoFlo Astrios EQ is designed to allow you to work with the greatest possible range of biological and non-biological particles, and you can fine-tune the instrument to suit your application. Adjust pressures to handle a wide range of cell types, improve cell vitality without compromising sort speed, and keep your cells alive and running with temperature and agitation options.

The MoFlo Astrios EQ is designed so that your application determines your instrument parameters, not the other way around. This flexibility enables you to conduct a wide range of single-cell applications, including:

  • Circulating Tumor Cells (CTCs)
  • Drug discovery applications
  • Rare event analysis
  • Stem cells
  • Neuronal cells
  • Fluorescent protein reporter selection
  • Ecological and physiological microbes (yeasts and bacteria)

In conventional cell sorters, there is a three-way trade-off between sort speed, sort purity and sort yield. The MoFlo Astrios EQ Mixed Mode sorting allows selecting for purity in one sort-direction and yield in another, enabling simultaneous recovery of virtually all the target cells that would otherwise be lost to achieve that high purity. This enables you to sort faster without losing extremely valuable cells, and without sacrificing purity of the principal sort.

Perform 6-way sorting without compromise:

• Save time by selecting more populations at once
• Save precious sample with our unique mixed-mode sorting capability 

Break free from cuvette sorting restrictions:

• Analyze heterogeneous populations with confidence — no differential velocities based on particle size; eliminate the need for Area: Height scaling and provides laser-delay optimization
• Maintain consistent performance with less maintenance — no concern about burn-in of debris on cuvette
• Allow biological demands to dictate sorting conditions — select from 70 and 100 micron nozzle sizes, to precisely accommodate cells from practically any tissue you want using Intellisort II to automatically maintain drop delay

See more with enhanced Forward Scatter (eFSC):

Look at data differently with 2 forward scatter PMTs

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Dual eFSC channels allow you to apply different application-specific masks for different particle types, or simply use different neutral density filters to allow a greater overall particle size range to be measurable simultaneously. The top histogram was measured on eFSC2 without any neutral density (ND) filter, to widen the separation between noise and 0.2 and 0.3 μm beads. The middle and lower histograms show the eFSC1 data (acquired with an ND1 filter) with different y-axis scales to better display the larger particle peaks. The entire dynamic range from 0.2 to 30 μm is easily made visible when different ND filters are used on each eFSC parameter.

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