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Beckman Coulter

CytoFLEX DEMO lehetőséggel

CytoflexMPL_01
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Ultra-flexible, high performance CytoFLEX system brings you easily upgradeable detection capabilities for up to 3 lasers and 13 color research flow cytometry right on your bench top.

ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK!

Providing quality and performance at any configuration, the CytoFLEX system provides powerful sensitivity and resolution for the simple to the most challenging applications. CytoFLEX delivers and surpasses capabilities expected in top tier analyzers, with excellent performance and nanoparticle resolution.

CytoFLEX is:

  • Powerful performance in all configurations
  • 21 available flexible configurations (up to 3 Lasers, 13 Colors)
  • Future proof solution with built in system flexibility for easy upgrade options
  • Superior sensitivity and resolution for excellent fluorescence and nanoparticle detection
  • Simple yet elegant design that is compact and reliable
  • CytExpert experiment based software for easy multicolor setup and QC
  • Easy to learn, easy to operate, easy to maintain
  • Versatile Plug-n-play filters –bandpass-only light collection
  • Integrated optics for alignment stability
  • Accommodates 12x75mm and microtiter tubes
  • Customer Installable – be ready to run in minutes not hours
  • Compact – Fits on your benchtop <50lbs, 16” x 16” x 13”

 

CytoFLEX filter configurations 
CytoFlex - Sensitivity
 

Sensitivity

CytoFLEX delivers and surpasses capabilities expected in top tier analyzers, with excellent sensitivity in all scatter and fluorescence.

The novel patent-pending optical design of the CytoFLEX research flow cytometer optimizes excitation from up to 3 lasers (488nm, 638nm, 405nm) and light collection efficiency. With unique flow cell design and integrated optics, the innovative, patent-pending Wavelength Division Multiplexing (WDM) detection module includes solid-state, high efficiency, low-noise detectors for excellent performance. The WDM’s bandpass only design also provide flexibility with a variety of configurations from 4 to 13 colors and easily changeable bandpass filters.

CytoFlex - Rainbow

Resolution

Nano Particle Detection

When equipped with a violet laser, the CytoFLEX can be set up to measure Side Scatter from 405nm for enhanced nanoparticle detection. Mie theory predicts that the scattering cross section of a particle, and thus its scattering intensity, depends on the wavelength of light, the angle of collection, and the size, shape, and refractive index of the particle. All other factors being equal, using a shorter illumination wavelength will result in an increase in scattering cross section, and thus more scattered light. The CytoFLEX offers light scattering parameter measurements from the 488nm laser light, and optionally from the 405nm laser to enhance small particle detection.

CytoFlex - Nano Particle Detection

Custom Configurations Available

The CytoFLEX provides built in upgradability for up to 13 colors affording you the capability to adapt to your changing applications needs without compromising your budget today. You can upgrade your configuration in less than a day with assurance that all CytoFLEX instruments have been fully tested in the maximum configurations available.


CytoFLEX - Custom Configurations Available

CytoFlex - Computer
 

For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Class 1 Laser Product 

Műszaki specifikációk

The CytoFLEX flow cytometer is designed to deliver superior performance, flexibility, and ease of operation for research applications. Simplified system setup, data acquisition, analysis, and export of experimental results are integrated into a complete workflow solution with CytExpert software.

The CytoFLEX system features a compact footprint, integrated detection optics and lasers, and a simplified, highly reliable fluidics system. The CytoFLEX flow cytometer is easily installed and upgradeable.Twenty one (21) standard configurations are available to provide the ultimate in application flexibility with up to three lasers and 15 parameters. Together, the CytoFLEX system and CytExpert software brings high performance flow cytometry to a variety of researchers with simple to complex needs.

 

CytExpert provides software innovation using intuitive workflow and novel tools to facilitate complex flow cytometric application setup.

Intuitive GUI for novice and flow expert

  • Experiment-based workspace design
  • FCS 3.0 compliant data files
  • Factory default instrument settings
  • Linear detector gain control
  • Gain-independent real-time compensation

    CytoFlex - Data & Stats
     

Compensation Library

  • Novice to experienced cytometrists can not only learn and operate the system quickly but can confidently set up high color applications with the innovative and intuitive software using the unique compensation library.
  • Store a repository of compensation spillover values of dyes in a library to easily determine the correct compensation matrix with new gain settings.

    CytoFlex - Comp Library Web
     

Simplified daily QC

  • Consistent performance across platform Levey-Jennings tracking of QC results over time.

    CytoFlex - Configuration Chart
     

CytExpert Plot Types

CytExpert software offers flexible analysis templates and plotting tools for easy and efficient data analysis.

CytoFlex - Software
Videók, ismertetők

CytoFLEX brochure

 

WEBINAR: Extracellular Vesicle Detection and Analysis via Flow Cytometry

Az elmúlt évtizedben megélénkült az érdeklődés a szekretált membrán vezikulák, összefoglaló néven  extracelluláris vezikulák (EV) iránt, hiszen EV szekréciót írtak le többek között hematológiai, kardiovaszkuláris, neurológiai és autoimmun kórképekben, valamint daganatos megbetegedésekben is. Az extracelluláris vezikulák tanulmányozásának egyik legfontosabb eszköze - a mikroszkópia mellett - a flow citometria, mindazok ellenére, hogy a hagyományos műszerekkel az EV populációnak csak kis része volt detektálható mérettartományú. A Beckman Coulter készülékekben alkalmazott új technológiai megoldásoknak köszönhetően - mint például az AstrioEQ sejtszorterben a speciális FSC detektálás, vagy az új CytoFLEX készülékben a Violet SSC - a citométerek és szorterek egyre inkább alkalmasak a szubcelluláris mérettartományú részecskék vizsgálatára. Az új eszközökkel elérhető ismeretek előrelépést hozhatnak a fent említett betegségek patogenezisének megértésében, emellett új diagnosztikai és terápiás lehetőségeket is teremthetnek, ezért a webinar témája nagy érdeklődésre tarthat számot.

Web: http://labroots.com/webinar/id/194

Időpont: Jan 20 2016 | 5:00 PM CET

Előadók: Vasilis Toxavidis, Resource Director Flow Cytometry Core, Beth Israel Deaconess Medical Center/Harvard Stem Cell Institute és John Tigges, Technical Director/Manager, Flow Cytometry Core, Beth Israel Deaconess Medical Center/Harvard Stem Cell Institute 

WEBINAR: A proposed phenotyping method for Human Innate Lymphoid Cells (ILCs) using flow cytometry

A Beckman Coulter szakértői csapatának tagja előadásában az immunológia egyik izgalmas területét, a veleszületett limfoid sejtek (ILC) témakörét járja körül: ismerteti a legfrissebb irodalmi adatokat, kitérve a kapuzási stratégiára és a megfelelő kompenzáció gyakorlati szempontjaira is. 

Web:   https://www.labroots.com/ms/webinar/id/189/beckman-olivier-dec18

Előadó: Olivier Jaen, PhD, Flow Cytometry Marketing Manager and Application Scientist, Beckman Coulter Emerging Market Europe Asia India

WEBINAR: Advanced sensitivity and resolution in flow cytometry through innovation

A flow citometria világszerte ismert szaktekintélye, J. Paul Robinson a BC új flow citométerében, a CytoFLEX készülékben alkalmazott innovatív technikai megoldásokról tart előadást.

Győződjön meg ön is a CytoFLEX kimagasló érzékenységéről és detektáló képességéről! Az érdeklődőknek cégünk DEMO lehetőséget biztosít!

Web: https://www.labroots.com/ms/webinar/id/188/beckman-Robinson-dec4

Elődadó: J. Paul Robinson, PhD, SVM Professor of Cytomics and Professor of Biomedical Engineering, Weldon School of Biomedical Engineering at Purdue University

Applikációk

The Unique Peristaltic Sample Delivery System of the CytoFLEX Analyzer Enables Optimized Measurements

Generation of fluorescent antibody or genetic labels to identify hormone and neurotransmitter receptoractivity can be difficult and time consuming. A useful alternative is recording physiological changes inresponse to agonist binding its cognate receptor, many of which are G-protein coupled. When an agonist binds a G-protein coupled receptor, it triggers a quick cascade of events that often results in a transient release of calcium from intracellular stores. Alterations in transient intracellular calcium ([Ca2+]i) levels have been used previously in flow cytometry to identify functional receptor expression in cellular subpopulations [2], here we show that peristaltic sample delivery of the new CytoFLEX analyzer is particularly well suited to agonist-based calcium studies. Using the ester based, green fluorescent calcium indicator, Fluo-4 AM (Life Technologies), [Ca2+]i changes were measured in HEK-293 cells in response to ATP stimulation. Simple plumbing modifications to the CytoFLEX allowed easier access to the sample tube for agonist application; further modifications were made to implement a “stop time” technique. By using response to agonist as our physiological criteria, we have fundamentally enabled receptor identification and conclusively demonstrated its functionality.


CytoFLEX Instrument Evaluation Using Biological Specimens

Evaluation of the Xitogen flow cytometer, by independent research flow cytometry laboratories and Beckman Coulter senior research and development scientists, was performed using flow cytometers equipped with 12 parameters and 9 fluorescent detectors. Instruments were equipped with the enhanced Side Scatter channel (VSSC) option. Evaluation of biological specimens included fresh human whole blood, human leukocyte control cells, and smaller cells and particles, such as RBCs, platelets, microbes, liposomes, and vesicles.


Set-Up Of The CytoFLEX For Extracellular Vesicle Measurement

The measurement and the characterization of Extracellular Vesicles (EV) have been of growing interest over the last 20 years. Flow cytometry instruments were not the most appropriate way to analyse these particles as the optical resolution of instruments was insufficient to detect particles below 250nm. However, the Beckman Coulter CytoFLEX* now offers the ability to measure EV down to at least 150nm and allows the detection of their cellular origin using up to 13 fluorescence parameters. Regardless of the technical improvements the set-up of the instrument is still a critical point and several requirements need to be met which are illustrated here.

Extracellular vesicles are a heterogeneous cell-derived particle population in a size range between 50nm to 1,000nm. There is a growing interest not only from academic research groups to determine EV in several fluids such as cell culture supernatant, in plasma samples or in whole blood but also in clinical research since it has been shown that the measurement of microparticles (MPs)1 might be of clinical relevance. The methods to identify EV are many and involve high speed centrifugation, Western blotting, proteomics, electron microscopy, imaging methods and flow cytometry. Methods for the detection of EV by flow cytometry have been developed in the last years and special attention has been paid to standardization protocols. Compared
with other methods, flow cytometry has the big advantage that EV can be detected as rare events, in high numbers and by antigens on the surface, which characterize their cellular origin.


Andreas Spittler, MD, Associate Professor for Pathophysiology, Medical University of
Vienna, Core Facility Flow Cytometry & Department of Surgery, Research Laboratories

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