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Molecular Devices

SpectraMax iD5 Multi-Mode Microplate Reader

Your All Access Pass to Unleash Your Brilliance
The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. In addition, the SpectraMax iD5 can be expanded to include bottom-read luminescence, TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection.
With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD5 is your all access pass to helping you unleash your brilliance.

ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK!

 

Key Features

  • Easily set up protocols, run experiments, view tutorial videos, and control user access with the intuitive SoftMax Touch Software for the touchscreen
  • Automatically identify filter sets using NFC functionality, simplifying workflows and eliminating confusion
  • Perform western blot detection with your microplate reader
  • Push data to workstations using QuickSync, eliminating the need to retrieve data from the instrument
  • Detect assay signal in the best way using the superior hybrid monochromator- and filter-based optical system
  • Run injector assays with SmartInject™ Technology, ensuring complete reagent mixing and rapid signal development

 

AUTOMATIC, NFC-ENABLED FILTER IDENTIFICATION

SpectraMax iD5 Automatic, NFC-Enabled Filter Identification

Using built-in near-field communication (NFC) tags, the SpectraMax iD5 plate reader automatically detects the identification code of the filter to recognize the slide and filter configuration, eliminating confusion and simplifying your filter workflow.

WESTERN BLOT CAPABILITY

SpectraMax iD5 Plate Reader with western blot capability

Protein detection is an important task for pharmaceutical and clinical research, and Western Blots are one of the most common methods employed for this purpose. The SpectraMax iD5 plate reader, utilizing an optional enhanced TRF detection module, is capable of scanning and analyzing your membranes for western blot data. The Europium-incubated membranes are resistant to photo bleaching and allow you to read the membranes without loss of signal for longer periods.

HYBRID MICROPLATE READER

Molecular Devices Hybrid Monochromator and Filter Based Microplate Reader

Detect assay signal the best way using the hybrid monochromator- and filter-based optical system.

 


QUICKSYNC DATA PUSH TECHNOLOGY

Reduce the amount of time you spend collecting data and increase your productivity. The SpectraMax iD5 reader allows you to interact with your data any way you want from anywhere you want. View your data quickly using the large touchscreen interface, export your data to a USB drive for analysis in the program of your choice, or analyze your data using the industry’s leading data acquisition and analysis tool, SoftMax Pro 7 Software. The SpectraMax iD5 reader also features network connectivity that allows you to walk away from the instrument to focus on additional research. Data is automatically delivered to any workstation on the same network, eliminating the need to physically retrieve data from the instrument.

SpectraMax iD5 Reader Quicksync Data Push Technology


NFC TOUCHSCREEN

SpectraMax iD5 with NFC Touchscreen

The innovative SpectraMax iD5 is the first five mode microplate reader featuring near-field communication (NFC) technology for touchscreen personalization, user acess control, and filter identification.

FLEXIBLE TEMPERATURE CONTROL
Flexible temperature control up to 66 degrees celsius

Simple-to-use temperature control allows you to adjust your experiment’s conditions from ambient up to 66°C, expanding your laboratory’s capabilities to include temperature sensitive assays.

DUAL AUTO INJECTORS

Dual Auto Injectors in SpectraMax iD5

The SpectraMax iD5 plate reader is injector-ready allowing you to expand your lab’s capabilities to include flash applications such as dual luciferase and ATP assays. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 μL), overflow protection, and ensures equal mixing across the plate for high-precision experiments.

Műszaki specifikációk

 

General specifications

Dimensions (in.) 15.79 (H) x 20.94 (W) x 23.54 (D)
Dimensions (cm) 40.1 (H) x 53.2 (W) x 59.8 (D)
Weight 88.1 lbs. (40 kg)
Power requirements 100–240 VAC, 2 A, 50/60 Hz
Robotic compatible Yes

General performance

Plate formats 6 to 384 wells 
Light source Xenon flash lamp
Reading capabilities Microplates, cuvettes (via adapter)
Detectors Photo Multiplier Tube and Photodiode
Shaking Linear, orbital, and double orbital
Temp. control 5°C above ambient to 66°C1Œ
Temp. uniformity ± 0.75°C
Temp. accuracy ±1°C at 37°C set point
Spectral scanning Abs, FI, Lum, TRF
Endpoint reading Abs, FI, Lum, TRF, FP, FRET, TR-FRET
Kinetic reading Abs, FI, Lum, TRF, FP, FRET, TR-FRET
Well scanning Over 20 by 20
Wavelength selection 1.0 nm increments

Standard read times (minutes:seconds)

 

96 wells

384 wells

Absorbance 0:30 1:30
Fluorescence intensity2 0:30 1:30
Luminescence2 0:30 1:30
Time-Resolved Fluorescence 0:30 1:30
Fluorescence Polarization 1:00 3:00

Absorbance photometric performance

Wavelength range 230–1000 nm
Wavelength bandwidth 4.0 nm full width half maximum
Wavelength accuracy ± 2.0 nm across wavelength range
Wavelength repeatability ± 1.0 nm
Photometric range 0–4.0 OD
Photometric resolution 0.001 OD
Photometric accuracy < ±0.010 OD ±1.0%
0–3 OD VIS, 0–3 OD UV 
Photometric precision < ±0.010 OD ±1.0%
0–3 OD VIS, 0–3 OD UV
Stray light < 0.05% @ 260 nm, 280 nm

Fluorescence intensity performance

Wavelength range (EX mono) 250–830 nm
Wavelength range (EM mono) 270–850 nm
Wavelength range (Filters) See accessory list
Wavelength selection (mono) 1.0 nm increments
Dynamic range > 6 logs

Optimized Top sensitivity (fluorescein)

  Monochromator Filters
96 wells 1 pM 0.3 pM
384 wells 1 pM 0.5 pM

Optimized Bottom sensitivity (fluorescein)

  Monochromator Filters
96 wells 2 pM 2 pM
384 wells 2.5 pM 2.5 pM

Fluorescence polarization performance

Wavelength range (EX mono) (300-)3 400-750 nm, 1.0 nm increments
Wavelength range (EM mono) (300-)3 400-750 nm, 1.0 nm increments
Wavelength range (Filters) See accessory list

Detection limit4

  Monochromator Filters
96 wells 2 mP @ 10 nM 1 mP @ 1 nM
384 wells 2 mP @ 10 nM 2 mP @ 1 nM
Measurement range5 Delta > 200 mP Delta > 320 mP

Luminescence performance

Wavelength range 300–850 nm
300-650 nm for "All Wavelengths" setting
Wavelength selection Choice of simultaneous detection of all wavelengths or selection in 1.0 nm increments
Dynamic range > 7 decades
Cross-talk < 0.1% in white 96- and < 0.2% in 384-well microplates
Detection limit 20 amol ATP ("Flash" luminescence using Promega ENLITEN® ATP Assay System)

Time-Resolved Fluorescence performance

Wavelength range (EM mono) 450-750 nm
Wavelength range (EX filter) 350 nm
Wavelength range (EM filter) 490 nm (Terbium), 616 nm (Europium)
For other filters, please see accessory list
LInear dynamic range Up to 5 logs
Detection limit
  96 wells (white) 384 wells (white)
Standard TRF 30 fM Europium
(6 amol/well)
30 fM Europium
(3 amol/well)
Enhanced TRF Module (Optional) 10 fM Europium
(2 amol/well)
10 fM Europium
(1 amol/well)

Injector system with SmartInject Technology (optional)

Injectors 2
Read modes Absorbance, fluorescence, luminescence
Dispense accuracy ± 5% at 100 μL
Dispense precision

CV ≤ 2% at 100 μL

Dead volume Injector tubing: 250 μL
< 10 μL with Reverse Prime function
ATP Concentration GraphAn ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD5 reader. Standard concentrations ranged from 1x10-11 M to 1x10-6 M. A wide linear dynamic range ensures accurate assay results across a broad span of sample types. Standards were plotted using a log-log curve fit in SoftMax Pro Software.
DNA Standards GraphThe SpectraMax iD5 reader is fully compatible with the SpectraDrop Micro-Volume Microplate, enabling quantitation of precious lowvolume samples. 4-uL DNA standards from 2 ng/μL to 1000 ng/ μL were read in absorbance detection mode with a preconfigured protocol in SoftMax Pro Software. Performance matching the 2 ng/μL sensitivity specification is demonstrated here.
  1. For > 66°C, minimum 25°C ambient temperature is required.
  2. 10 msec integration time for fluoresence measurement. 100 msec integration time for luminescence for a 96-well plate and 40 msec integration time for a 384-well plate.
  3. Requires optional UVIS polarizer
  4. 1x Stdev Fluorescein replicates [mP]
  5. ThermoFisher P3088, FP One-Step reference kit
 

 

Applikációk

 

The SpectraMax iD5 is a hybrid monochromator- and filter-based, five-mode microplate reader that allows you to expand the boundaries of your research to include a wide variety of applications.

  • Cell biology
  • Protein biology
  • Apoptosis
  • BRET
  • Cellular imaging assays
  • Cell cycle analysis
  • Cell proliferation, viability, cytotoxicity
  • Enzyme activity
  • Epigenetics
  • Flash based applications including dual luciferase and ATP assays
  • Fluorescent protein expression
  • FRET
  • Genotyping
  • GPCRs
  • HTRF®
  • Immunoassays
  • Ion channels
  • Membrane potential assays
  • Micro Volume (Low volume) Applications
  • Molecular interactions
  • Nuclear receptor
  • Nucleic acid and Protein quantitation
  • Reporter assay
  • Time-Resolved Fluorescence (TRF)
  • Western blot detection
  • Cell line development
  • Antibody discovery
  • Screening
  • Genomics

 

Videók, ismertetők

Five-Mode Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability

 
SpectraMax iD5 Multi-Mode Microplate Reader Brochure
SpectraMax iD5 Multi-Mode Microplate Reader Brochure

Five-mode microplate reader with automatic filter identification and western blot capability The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. In addition, the SpectraMax iD5 reader can be expanded to include bottom-read luminescence, TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD5 reader is your all access pass to helping you unleash your brilliance.

Click here to download the product brochure >>

Application Note: Detect inflammatory cytokines at picogram levels with Sword ELISA Boosters

Traditional ELISA methods using the horseradish peroxidase (HRP) substrate 3,3’,5,5’- tetramethylbenzidine (TMB) often fail to detect low abundance analytes, such as inflammatory cytokines. The Sword ELISA Booster, a next-generation ELISA detection technology that directly replaces the detection reagents in a traditional, gold-standard ELISA, increases sensitivity up to 30 fold.

This enhanced assay allows you to detect lower levels of these cytokines and advance your understanding of diseases associated with these mediators of inflammation. Here, we demonstrate how three SpectraMax® microplate readers we tested met the criteria for sensitivity outlined for the Sword ELISA Boosters.

Click here to download the application note >>

Application Note: Monitor NF-κB activation with a sensitive dual reporter assay on the SpectraMax iD5 microplate reader

SpectraMax iD5 Application Note - Monitor NF-κB activation with sensitive dual reporter assay

Reporter genes are valuable tools for studying gene expression, serving as surrogates for genes involved in various signaling pathways and disease conditions. Luciferases are the most common reporter genes, as they are easily detectable using luminometers or luminescence microplate readers, and the low luminescent background present in cells enables high assay sensitivity. Firefly luciferase is typically used as a readout for expression of a gene of interest. Luciferase from the sea pansy Renilla reniformis is often used in multiplexed luciferase assays as a second reporter to normalize for transfection efficiency, cell viability, and other factors that may vary among assay samples. Here we show how the DuoLuc reporter assay and SpectraMax iD5 reader are used to detect nuclear factor-κB (NF-κB) activation in a mammalian cell model. NF-κB is a ‘master regulator’ of gene expression for many cellular processes including inflammation, immunity, differentiation, proliferation, and apoptosis. 

Click here to download the application note >>

 

View SpectraMax iD3 Tutorial Videos
 

Getting to know your new SpectraMax iD5 Multi-Mode Microplate Reader

View a brief overview of the features and benefits of your new SpectraMax iD5 Multi-Mode Microplate Reader.
Click here to view >>

 

Personalizing your new SpectraMax iD5 Multi-Mode Microplate Reader

Learn how to personalize your SpectraMax iD5 Multi-Mode Microplate Reader.  Create user profiles and pair them to NFC tags to easily pull up saved protocols, and experiment results.
Click here to view >>

 

Starting a plate read on your new SpectraMax iD5 Multi-Mode Microplate Reader

Learn how to quickly and easily start a plate read using the high-resolution touchscreen interface.
Click here to view >>

 

Viewing results on your new SpectraMax iD5 Multi-Mode Microplate Reader

Tutorial on how to quickly and easily view your results on the SpectraMax iD5 Multi-Mode Microplate Reader.
Click here to view >>

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