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Molecular Devices

SpectraMax iD3 Multi-Mode Microplate Reader

Identifiably Different

The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to perfectly match your needs.




The SpectraMax iD3 Multi-Mode Microplate Reader features a large, high-resolution touchscreen with an embedded software package allowing you to set up custom protocols, take advantage of preconfigured protocols, and run your experiment without the need for a dedicated computer workstation.



A built-in near-field communication (NFC) reader enables you to pull up your custom protocols with a single tap, saving you precious time. Pair NFC Tags with specific user profiles to gain access to personalized protocols and experiment results eliminating the need to scroll through dozens of files.



The superior optical system includes a xenon flash lamp and features an ultra-cooled photomultiplier tube (PMT) to -5°C that reduces background noise for excellent sensitivity and a wide dynamic range.



Simple-to-use temperature control allows you to adjust your experiment's conditions from ambient up to 65°C, expanding your laboratory's capabilities to include temperature sensitive assays.



View your data quickly using the large touchscreen, export to a USB drive, or analyze your data using the industry-leading data acquisition and analysis tool, SoftMax Pro 7 Software. QuickSync technology allows you to walk away from the SpectraMax iD3 to focus on additional research. Data is automatically delivered to your workstation, eliminating the need to physically retrieve your data from the instrument.



Featuring orbital shaking, a four-monochromator optical pathway with high efficiency gratings, well scanning up to a 20x20 read matrix, spectral scanning and detection of plate formats from 6- to 384-wells, the SpectraMax iD3 reader is the complete solution to all your research needs.



Expand your lab’s capabilities to include flash applications such as dual luciferase and ATP assays with the SpectraMax iD3 injector system with SmartInject™ Technology ensuring equal mixing across the plate for high-precision experiments. Engineered for high performance, the dual injector system can be used with any read mode and includes safeguard features such as overflow protection to save precious reagents and maximize performance for those critical experiments in your lab.


SoftMax® Pro GxP Compliance Software extends Molecular Devices leading data acquisition and analysis solution into regulated laboratories working under GMP, GLP, 21 CFR Part 11, and o





Applications of SpectraMax iD3 and iD5 Multi-Mode Microplate Readers

  • Cell Viability

    Cell Viability

    Cell viability can be assessed by examining parameters such as cell membrane integrity or the activity of cellular enzymes. On a microplate readers, these parameters can be detected using fluorescent reagents. For example, a red cell-impermeant, DNA-binding dye only stains dead or dying cells whose membranes are compromised, while a green live-cell dye only fluoresces when metabolic enzymes are active.

    Read Application Note

  • DNA/RNA Quantitation

    DNA/RNA Quantitation

    The absorbance of a DNA sample measured at 260 nm on a spectrophotometer or microplate reader can be used to calculate its concentration. Absorbance quantitation works on samples ranging from about 0.25 ug/mL to about 125 ug/mL in a microplate format. Some instrumentation enables the quantitation of very small sample volumes, as little as 2 uL. When greater sensitivity is required, fluorescence methods allow quantitation of as little as a few picograms of DNA.

    Read Application Note

  • Western Blot Detection

    Western Blot Detection

    Western blotting is among the most common methods employed for the detection and quantitation of specific proteins. In this method, protein sample, e.g. from a cell lysate, is first separated by size on an SDS-PAGE gel. Proteins are then transferred to a nitrocellulose or PVDF membrane, which is probed with antibody specific to the protein of interest. Various techniques are used to detect proteins on western blot membranes including fluorescence and chemiluminescence.

    Read Application Note

  • HTRF


    HTRF®, an assay technology developed by Cisbio, combines standard FRET (fluorescence resonance energy transfer) with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. HTRF’s applications include assessment of small phosphorylated peptides, immunoassays for quantifying large glycoproteins, and indirect detection of tagged complexes such as CD28/CD86 via secondary antibodies. These homogeneous assays do not require wash steps and are amenable to small-volume microplate formats used in high-throughput screening.
    (This assay is suitable for SpectraMax® iD5 and SpectraMax® Paradigm readers.)

    Read Application Note

  • SpectraMax DuoLuc Reporter Assay High Resolution

    Reporter Gene Assay

    Reporter gene assays enable researchers to determine whether a gene is expressed and at what level. They are used to study signaling pathways, gene regulation, structure of regulatory elements, and much more. A reporter gene vector consists of a promoter or gene of interest, along with a reporter gene such as firefly luciferase, which codes for a protein whose activity can be easily measured. The vector is transfected into mammalian cells, and when the gene of interest is active in the cells, the reporter gene is also expressed and can be measured using appropriate detection reagents.

    Read Application Note



Videók, ismertetők



SpectraMax iD3 Application Note
One of the most popular methods for assaying cytotoxicity is fluorescence detection with a microplate reader. In this application note, we report the use of the EarlyTox™ Cell Viability suite of assay kits on the SpectraMax® iD3 Multi-Mode Microplate Reader.
Click here to download the application note >>

View SpectraMax iD3 Tutorial Videos

SpectraMax iD3 Video Tutorial
Getting to know your new SpectraMax iD3 Multi-Mode Microplate Reader

View a brief overview of the features and benefits of your new SpectraMax iD3 Multi-Mode Microplate Reader.
Click here to view >>

SpectraMax iD3 Personalization
Personalizing your new SpectraMax iD3 Multi-Mode Microplate Reader

Learn how to personalize your SpectraMax iD3 Multi-Mode Microplate Reader.  Create user profiles and pair them to NFC tags to easily pull up saved protocols, and experiment results.
Click here to view >>

SpectraMax iD3 Plate Read Touchscreen Interface
Starting a plate read on your new SpectraMax iD3 Multi-Mode Microplate Reader

Learn how to quickly and easily start a plate read using the high-resolution touchscreen interface.
Click here to view >>

SpectraMax iD3 Output Measurement Tutorial
Viewing results on your new SpectraMax iD3 Multi-Mode Microplate Reader

Tutorial on how to quickly and easily view your results on the SpectraMax iD3 Multi-Mode Microplate Reader.
Click here to view >>




Műszaki specifikációk

General specifications

Dimensions (in.) 15.79 (H) x 20.94 (W) x 23.54 (D)
Dimensions (cm) 40.1 (H) x 53.2 (W) x 59.8 (D)
Weight 88.1 lbs. (40 kg)
Power requirements 100–240 VAC, 2 A, 50/60 Hz
Robotic compatible Yes

General performance

Plate formats 6 to 384 wells 
Light source Xenon flash lamp
Reading capabilities Microplates, cuvettes (via adapter)
Detectors Photo Multiplier Tube and Photodiode
Shaking Orbital, Infinity
Temp. control 5°C above ambient to 65°CŒ
Temp. uniformity ± 0.75°C
Temp. accuracy ±1°C at 37°C set point
Spectral scanning Abs, FI, Lum
Endpoint reading Abs, FI, Lum
Kinetic reading Abs, FI, Lum
Well scanning Over 20 by 20 in all modes
Wavelength selection 1.0 nm increments

Standard read times (minutes:seconds)


96 wells

384 wells

Absorbance 0:25 1:25
Fluorescence intensity 0:17 0:53
Luminescence 0:26 1:01

Absorbance photometric performance

Wavelength range 230–1000 nm
Wavelength bandwidth 4.0 nm
Wavelength accuracy ± 2.0 nm
Wavelength repeatability ± 1.0 nm
Photometric range 0–4.0 OD
Photometric resolution 0.001 OD
Photometric accuracy < ±0.010 OD ±1.0%, 0–3 OD
Photometric precision < ±0.003 OD ±1.0%, 0–3 OD
Stray light < 0.05% @ 230 nm

Fluorescence intensity performance

Wavelength range 250–850 nm
Wavelength selection 1.0 nm increments
Dynamic range > 6 decades

Optimized Top sensitivity (fluorescein)

96 wells 1 pM
384 wells 1 pM

Optimized Bottom sensitivity (fluorescein)

96 wells 2 pM
384 wells 2.5 pM

Luminescence performance

Wavelength range 300–850 nm
Wavelength selection Choice of simultaneous detection of all wavelengths or selection in 1.0 nm increments
Dynamic range > 7 decades
Cross-talk < 0.1% in white 96- and < 0.2% in 384-well microplates

Optimized Sensitivity (ATP-Glow)

96 wells 2 pM
384 wells 4 pM

Injector system with SmartInject Technology (optional)

Injectors 2
Read modes Absorbance, fluorescence, luminescence
Dispense accuracy ± 5% at 100 μL
Dispense precision

CV ≤ 2% at 100 μL

Dead volume Injector tubing: 250 μL
< 10 μL with Reverse Prime function
Detection limit 20 amol ATP ("Flash" luminescence using Promega ENLITEN® ATP Assay System)

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