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PrimeTime® qPCR probe-based assays |
Select from various combinations of dyes and quenchers
Choose from 3 reactions scales - 100, 500 or 2500 reactions
Order in tubes or 96-well plates
PrimeTime qPCR Primer Assays are provided without probe for intercalating dye experiments.
Receive 5 nmoles each of forward and reverse primers mixed, sufficient for 500 reactions (20 µL)
Order in tubes or 96-well plates
Choose from 3 reactions scales - 100, 500 or 2500 reactions
Order in tubes or 96-well plates
PrimeTime qPCR Primer Assays are provided without probe for intercalating dye experiments.
Receive 5 nmoles each of forward and reverse primers mixed, sufficient for 500 reactions (20 µL)
Order in tubes or 96-well plates
ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK!
FIGYELEM!
IDT termékek szállítási költségei 2024-től:
A szintézis helyétől, valamint a szállítási körülményektől függően az alábbi költségek kerülnek felszámításra:
- Szállítási és kezelési költség – Belgiumból érkező csomag esetén: nettó 5 850 Ft
- Szállítási és kezelési költség – USA-ból érkező csomag esetén: nettó 32 000 Ft
- Szárazjeges szállítási költség – Belgiumból érkező szárazjeges csomag esetén: nettó 30 000 Ft
- Szárazjeges szállítási költség – USA-ból érkező szárazjeges csomag esetén: nettó 97 000 Ft
- Oldatok szállítási költsége: mennyiség és súly függvénye, kérjen ajánlatot a Bio-Science Kft.-től!
Általános tájékoztató az IDT USA gyártási központjából érkező „custom” termékcsoportokról:
szintetikus biológiai termékek:
- Gének
- gBlocks, eBlocks
- Ultramerek
- RNS-oligók
- CRISPR guide RNS-ek
- oPools
qPCR próbák:
a szintézis helye a szekvencia komplexitásától, valamint a választott festék/ quencher kombinációtól függ
szolgáltatások:
- PAGE tisztított termékek
stock termékek:
nem áll teljes lista rendelkezésre, kérjen ajánlatot a Bio-Science Kft.-től!
Additional Benefits and Features
- Primer and probe sequences provided with each order:
- To assist with compliance with MIQE Guidelines
- Allow you to fully analyze and potentially troubleshoot your experiments
- Primer and probe sequences provided with each order:
- Receive multi-reaction plates, depending on scale ordered - 100, 500, or 2500 reactions [20 µL] per well
- Order assays with probes that have different dye–quencher combinations in the same plate
- Generate your own replicate plates for lower cost per reaction
- Order a minimum of 24 assays or primer pairs per plate
PrimeTime® qPCR 5' Nuclease Assays
PrimeTime qPCR 5' Nuclease Assays are offered in three different sizes to meet any qPCR experimental need. In addition, for the Standard and XL sizes, selection of dye–quencher combination and primer-to-probe ratio can be specified to meet unique experimental demands. Assays consist of a forward primer, a reverse primer, and a qPCR probe all delivered in a single tube. Each oligonucleotide undergoes 100% QC by mass spectrometry and all QC results are provided free of charge to the customer on the IDT website.
- Primers and probe mixed and delivered in a single tube or plate well
- Online assay design tools provide guaranteed performance
- Available in 5 dye–quencher combinations and 3 reaction scales
- Choose ZEN™ Double-Quenched Probes in your Assay for superior performance compared to traditional dual-labeled probes
- MIQE Compliant: All primer and probe sequences are provided
- Available in tubes or 96-well plates
PrimeTime® qPCR 5' Nuclease Assays in Tubes
| No. of Reactions (20 µL) | Probe (nmoles) | Primers (nmoles)1 | |
|---|---|---|---|
| PrimeTime® Mini qPCR Assay |
100 | 0.5 | 1.0 |
| PrimeTime® Standard qPCR Assay |
500 | 2.5 | 2.5-10 |
| PrimeTime® XL qPCR Assay |
2500 | 12.5 | 12.5-50 |
PrimeTime® qPCR 5' Nuclease Assays in 96-Well Plates
| PrimeTime Assay Plates2 | Reactions (20 µL) | Probe (nmoles) | Primers (nmoles)2 |
|---|---|---|---|
| PrimeTime® Assay Plate Mini |
100 | 0.5 | 1.0 |
| PrimeTime® Standard qPCR Assay |
500 | 2.5 | 2.5-10 |
| PrimeTime® XL qPCR Assay |
2500 | 12.5 | 12.5-50 |
1 The primer-to-probe ratio can be specified by the customer (except for PrimeTime Mini).
2 A minimum order of 24 assays is required per plate.
Available Dye and Quencher Combinations
for PrimeTime® qPCR 5' Nuclease Assays
| 5' Dye | 3' Quencher | Mini | Standard | XL |
|---|---|---|---|---|
| FAM | ZEN/Iowa Black FQ* |  |
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| FAM | TAMRA | |
|
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| HEX | ZEN/Iowa Black FQ* | |
|
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| TET | ZEN/Iowa Black FQ | |
|
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| Cy5 | Iowa Black RQ | |
|
*ZEN™/Iowa Black FQ is a Double-Quenched Probe which provides superior performance to traditional dual-labeled probes.
PrimeTime qPCR Primer Assays
PrimeTime® qPCR Primer Assays are predesigned qPCR assays used to detect genes in the human, mouse, and rat transcriptomes. These primer sets are ideal for use with SYBR® Green, EvaGreen®, and other intercalating dyes, where no probe is needed. PrimeTime qPCR Primer Assays are available in Standard Size, premixed and normalized to 5 nmoles per primer, and are shipped dried down. Primer sequences are provided upon order, and the primers ship in 2–3 business days.
- The same primer pairs found in the PrimeTime qPCR 5' Nuclease Assays
- Forward and reverse primers mixed and delivered in a single tube or plate well
- Ideal for use with SYBR® Green, EvaGreen, and other intercalating dyes, where no probe is needed
- Available in tubes, 96-well plates, or Matrix plates
PrimeTime® qPCR Primer Assays in Tubes
| Reactions (20 µL) | Quantity (nmoles) | |
|---|---|---|
| PrimeTime qPCR Primers | 500 | 5 |
PrimeTime® qPCR Primer Assays in Plates
| Reactions (20 µL) | Quantity (nmoles) | |
|---|---|---|
| PrimeTime™ Primer Plate | 500 | 5 |
Custom qPCR Probes
Double-quenched qPCR probes
While traditional probes have approximately 20–30 bases between the fluorophore and the quencher, the internal ZEN™ or TAO™ quencher decreases that length to only 9 bases. This shortened distance, particularly when combined with the traditional 3’ end quencher, leads to a much more thorough quenching with much lower background and enables the use of much longer probes for designing in AT-rich target regions. In addition to the significantly decreased background, the Double-Quenched Probes also have consistently reduced Cq values and improved precision when compared to traditional probes. Use of the Double-Quenched Probes can allow users to experience both increased sensitivity and precision in their qPCR experiments.
- ZEN Double-Quenched Probes
ZEN Double-Quenched Probes contain a 5’ fluorophore; 3’ IBFQ quencher; and proprietary, internal ZEN quencher. The most commonly supplied 5′ fluorophores includes FAM, Hex™, TET™, MAX™, or JOE. - TAO Double-Quenched Probes
TAO Double-Quenched Probes are available with a 5’ Cy® 5 fluorophore; 3’ IBRQ quencher; and proprietary, internal TAO quencher.
Choose from a wide variety of synthesis scales, dyes, and quenchers, including several license-free combinations. Prices and estimated turnaround times depend on the degree of complexity.
PrimeTime® qPCR Probes - Standard Level
The minimum guarantees are listed for probes that are 15–35 bases in length.
| 5' Reporter Dye(s) | Quencher | Synthesis Scale / Minimum Guarantee | ||
|---|---|---|---|---|
| FAM | ZEN / Iowa Black FQ * |
100 nmol / 15 nmol |
250 nmol / 25 nmol |
1 umol / 50 nmol |
| 5' Reporter Dye(s) | Quencher | Synthesis Scale / Minimum Guarantee | ||
|---|---|---|---|---|
| FAM | TAMRA | 100 nmol / 15 nmol |
250 nmol / 25 nmol |
1 umol / 50 nmol |
| Black Hole Quencher 1 |
100 nmol / 15 nmol |
250 nmol / 25 nmol |
1 umol / 50 nmol |
|
| HEX | ZEN / Iowa Black FQ * |
100 nmol / 15 nmol |
250 nmol / 25 nmol |
1 umol / 50 nmol |
| Black Hole Quencher 1 |
100 nmol / 15 nmol |
250 nmol / 25 nmol |
1 umol / 50 nmol |
|
| TET | ZEN / Iowa Black FQ * |
100 nmol / 15 nmol |
250 nmol / 25 nmol |
1 umol / 50 nmol |
| Black Hole Quencher 1 |
100 nmol / 15 nmol |
250 nmol / 25 nmol |
1 umol / 50 nmol |
|
| YAK | ZEN / Iowa Black FQ * |
100 nmol / 15 nmol |
250 nmol / 25 nmol |
1 umol / 50 nmol |
* ZEN/Iowa Black FQ is a double-quenched probe which provides superior performance compared to traditional single-quenched probes.
PrimeTime® qPCR Probes - Complex Level
The minimum guarantees are listed for probes that are 15–35 bases in length.
| 5' Reporter Dye(s) | Quencher | Synthesis Scale / Minimum Guarantee | ||
|---|---|---|---|---|
| FAM | TAMRA NHS Ester | N/A | 250 nmol / 8 nmol |
1 umol / 20 nmol |
| MAX NHS | ZEN / Iowa Black FQ * |
100 nmol / 2 nmol |
250 nmol / 8 nmol |
1 umol / 20 nmol |
| Black Hole Quencher 1 |
100 nmol / 2 nmol |
250 nmol / 8 nmol |
1 umol / 20 nmol |
|
| Cy3 | Iowa Black RQ | 100 nmol / 2 nmol |
250 nmol / 8 nmol |
1 umol / 20 nmol |
| Black Hole Quencher 2 |
100 nmol / 2 nmol |
250 nmol / 8 nmol |
1 umol / 20 nmol |
|
| TEX 615 | Iowa Black RQ | 100 nmol / 2 nmol |
250 nmol / 8 nmol |
1 umol / 20 nmol |
| Black Hole Quencher 1 |
100 nmol / 2 nmol |
250 nmol / 8 nmol |
1 umol / 20 nmol |
|
| Cy5 | Iowa Black RQ | 100 nmol / 2 nmol |
250 nmol / 8 nmol |
1 umol / 20 nmol |
| Black Hole Quencher 2 |
100 nmol / 2 nmol |
250 nmol / 8 nmol |
1 umol / 20 nmol |
|
| TAO / Iowa Black RQ * |
100 nmol / 2 nmol |
250 nmol / 8 nmol |
1 umol / 20 nmol |
|
| TYE 563 | Iowa Black RQ | 100 nmol / 2 nmol |
250 nmol / 8 nmol |
1 umol / 20 nmol |
| Black Hole Quencher 1 |
100 nmol / 2 nmol |
250 nmol / 8 nmol |
1 umol / 20 nmol |
|
| TYE 665 | Iowa Black RQ | 100 nmol / 2 nmol |
250 nmol / 8 nmol |
1 umol / 20 nmol |
| Black Hole Quencher 2 |
100 nmol / 2 nmol |
250 nmol / 8 nmol |
1 umol / 20 nmol |
|
| JOE NHS Ester | Iowa Black RQ | 100 nmol / 2 nmol |
250 nmol / 8 nmol |
1 umol / 20 nmol |
| Black Hole Quencher 1 |
100 nmol / 2 nmol |
250 nmol / 8 nmol |
1 umol / 20 nmol |
|
| TAMRA NHS Ester |
Iowa Black RQ | 100 nmol / 2 nmol |
250 nmol / 8 nmol |
1 umol / 20 nmol |
| Black Hole Quencher 2 |
100 nmol / 2 nmol |
250 nmol / 8 nmol |
1 umol / 20 nmol |
|
| ROX NHS Ester | Iowa Black RQ | 100 nmol / 2 nmol |
250 nmol / 8 nmol |
1 umol / 20 nmol |
| Black Hole Quencher 1 |
100 nmol / 2 nmol |
250 nmol / 8 nmol |
1 umol / 20 nmol |
|
| Texas Red-X NHS Ester |
Iowa Black RQ | 100 nmol / 2 nmol |
250 nmol / 8 nmol |
1 umol / 20 nmol |
| Black Hole Quencher 2 |
100 nmol / 2 nmol |
250 nmol / 8 nmol |
1 umol / 20 nmol |
|
* ZEN/Iowa Black FQ and TAO/Iowa Black RQ are double-quenched probes which provide superior performance compared to traditional single-quenched probes.
Small-scale qPCR probes
PrimeTime® Mini qPCR Probes
PrimeTime Mini qPCR probes are probes available on a small scale. The smaller scale and lower price makes them ideal for trying out a new probe, for screening the expression levels of many genes, or for testing the conversion to FAM/IBFQ and FAM/ZEN/IBFQ from other FAM-related quenchers. Mini probes are offered with a 5’ FAM and the option of a 3’ IBFQ quencher alone or in combination with the internal ZEN™ quencher. The estimated turnaround time is 3–5 days.
| 5' Reporter Dye(s) | Quencher(s) | Delivery Amount |
|---|---|---|
| FAM | ZEN / Iowa Black FQ * | 0.5 nmol |
PrimeTime® Eco qPCR Probes
PrimeTime Mini qPCR probes are probes available on a small scale. The smaller scale and lower price makes them ideal for trying out a new probe, for screening the expression levels of many genes, or for testing the conversion to FAM/IBFQ and FAM/ZEN/IBFQ from other FAM-related quenchers. Mini probes are offered with a 5’ FAM and the option of a 3’ IBFQ quencher alone or in combination with the internal ZEN™ quencher.
| 5' Reporter Dye(s) | Quencher(s) | Delivery Amount |
|---|---|---|
| FAM | ZEN / Iowa Black FQ * | 2.5 nmol |
* ZEN/Iowa Black FQ is a double-quenched probe which provides superior performance compared to traditional single-quenched probes.
For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.
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