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BIO SCIENCE
Thermo Scientific

NanoDrop 3300 Fluorospectrometer
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Fluorescence Made Compact and Simple

Thermo Scientific NanoDrop 3300 fluorospectrometers are used by life science laboratories to verify selective concentrations of DNA, RNA, and protein. Utilizing a patented sample retention system it minimizes the mass detection limit while increasing sensitivity, allowing measurement of samples as low as
1 picogram/µl of dsDNA. The flexibility of the NanoDrop 3300 allows users to work with multiple brands of assays, giving them the freedom to select the best assay for a specific workflow.

ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK!

 

NanoDrop 3300 Fluorospectrometer features:

  • Measures a wide variety of fluorescence assays:  RiboGreen, PicoGreen, GFP, BFP mutants, Hoechst, 4-MU, Quantum Dots, OPA, DyLite, AlexaFluor and many more
  • Sample size as small as 1μL
  • UV, blue and white LED excitation, covering a broad wavelength range
  • No filter changes and no expensive monochromator
  • Ability to run diverse and customizable applications
  • Ability to measure multiple fluorophores from a single sample
  • Spectral and quantitative data

 

 

How It Works


The sample retention system used by the Thermo Scientific NanoDrop 3300 Fluorospectrometer enables analysis of extremely small sample volumes (1-2 µl) without the use of cuvettes  or capillaries. Preparing the instrument for another measurement simply involves cleaning the sample off with an ordinary lab wipe. The system saves on expenses associated with disposables as well as on time and effort spent cleaning  quartz cuvettes.

 

With the apparatus arm open, a droplet of sample is pipetted onto the measurement pedestal.
When the apparatus is closed, the sample arm slightly compresses the droplet and a liquid column is drawn. Surface tension alone holds the sample in place for the spectral measurement. The spectral measurement is then made and quantification is made based a sample length of 1 mm.   

When the measurement is complete, the apparatus arm is opened and the sample is simply wiped from both the upper bushing and lower pedestal using an ordinary dry laboratory wipe.

Since the sample is not contained in a secondary vessel, the sample directly wets the system optics, reducing the variations resulting from changing and/or repositioning cuvettes. When the sample is removed, the optics can be easily cleaned making it possible to measure successive sample concentrations varying by more than 1000 fold in concentration with no carryover.

 

Software

Nucleic Acid Quantitation
  • dsDNA 33258 Hoechst dye
  • dsDNA PicoGreen® dye
  • Quant-It™ DNA BR
  • Quant-It™ DNA HS
  • RNA RiboGreen® dye
  • Sybr® Green I

Protein Quantification

  • Fluorescamine
  • FluoroProfile®
  • Fluoraldehyde™ OPA
  • Quant-iT™ Protein

Additional Capabilities

  • Fluorescence Profiler
  • Data Viewer
  • Custom Method Editor
  • Source Check

 

Other Fluorophores

  • Fluorescein, FITC, FAM
  • Cy3, Alexa Fluor® 555
  • Cy5, Alexa Fluor® 647
  • Quinine Sulfate
  • 4-methyl umbelliferone
  
  • DyLight 405
  • DyLight 488
  • DyLight 549
  • DyLight 633
  • DyLight 649
  • DyLight 680

Performance

Data from studies using the Thermo Scientific NanoDrop 3300 fluorospectrometer on many fluorophores is available below:

 

Műszaki specifikációk
Instrument Type   Fluorospectrometer
Minimum Sample Size 1 µl
Light Sources 3 light emitting diodes (LEDs)
Excitation Maxima UV: 365 nm
Blue: 470 nm
White 460-650 nm
Detector Type 2048 - element linear silicon CCD array
Wavelength Range 400-750 nm
Wavelength Accuracy 1 nm
Spectral resolution 8 nm (FWHM at Hg 546 nm)
Fluorescence Precision < 5% CV (10 nM fluorescein)
Detection Limit < 1 fmol fluorescein
Measurement Cycle Time 2 - 10 seconds
Dimensions (footprint) 14 cm X 20 cm
Weight 1.5 kg
Sample Pedestal Material of Construction 303 stainless steel and quartz fiber
Operating Voltage 5 vdc (supplied by USB port, no external power supply)
Operating Power Consumption 2 W
Standby Power Consumption 1 W
Software Compatibility Professional versions of Windows 7 (32 bit and 64 bit), Windows Vista (32 bit), Windows XP (32 bit)
UL/CSA and CE All units are approved to these standards
Applikációk
  • Measuring Enzyme Activity Using the Thermo Scientific NanoDrop 3300 Fluorospectrometer
  • A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice (JoVE Protocol Video)
  • Measuring Mouse GFR by FITC-Inulin using the Thermo Scientific NanoDrop 3300 Fluorospectrometer
  • Microvolume Quantification of Proteins by UV-Vis Absorbance or Fluorescence (Poster)
  • Using the NanoDrop 3300 Fluorospectrometer for Cancer Research Applications
  • Using the NanoDrop 3300 Fluorospectrometer For Micro-Volume Fluorescence/FRET Applications
  • Using the NanoDrop 3300 Fluorospectrometer For Nanoparticle/Quantum Dot Applications
  • Molecular Beacon Probes: Micro-Volume Fluorescence Measurement of HPLC Isolated Probes
Videók, ismertetők

Brochure

 

How It Works

See the NanoDrop 3300 in action

 

PUBLICATIONS

 

Its a question of conservation

Labnews.co.uk


The State of Microsample Quantitation

Genetic Engineering News, April 2007


Photonics Takes on the Challenge of Microanalysis

BioPhotonics International, January 2006


Diverse Fluorometers For Diverse Applications

Biocompare

 

CITATIONS

 

Click here to look for additional NanoDrop Fluorometer citations on Google Scholar

Please add additional key words to find your application of interest.


Crystal structure of the electron transfer complex rubredoxin rubredoxin reductase of Pseudomonas aeruginosa.

FAD fluorescence. Reference: Gregor Hagelueken, Lutz Wiehlmann, Thorsten M. Adams, Harald Kolmar, Dirk W. Heinz, Burkhard Tümmler, and Wolf-Dieter Schubert, Proc Natl Acad Sci U S A. 2007 Jul 24;104(30):12276-81. doi: 10.1073/pnas.0702919104.


Caulobacter crescentus as a whole-cell uranium biosensor

In cell GFP fluorescence. Reference: Hillson, Nathan J., Hu, Ping, Andersen, Gary L., Shapiro, Lucy Appl. Environ. Microbiol. 2007 73: 7615-7621, doi:10.1128/AEM.01566-07


MS-qFRET: A quantum dot-based method for analysis of DNA methylation.

Quantitation of DNA methylation by MS-qFRET measurements. Reference: Vasudev J. Bailey, Hariharan Easwaran, Yi Zhang, Elizabeth Griffiths, Steven A. Belinsky, James G. Herman, Stephen B. Baylin, Hetty E. Carraway, and Tza-Huei Wang Genome Res. 2009. 19: 1455-1461, doi: 10.1101/gr.088831.108


Behavior of fluorescent molecules bound to the interior of silica nanocapsules in various solvents.

Characterization of fluorescein and Dy485XL labeled silicon nanoparticles. Reference: Hirokazu Miyoshi, Yuki Matsuo, Yiyao Liu, Takao Sakata and Hirotaro Mori, Journal of Colloid and Interface Science Volume 331, Issue 2, 15 March 2009, Pages 507-513, doi:10.1016/j.jcis.2008.09.026


Expression of a class 1 hemoglobin gene and production of nitric oxide in response to symbiotic and pathogenic bacteria in Lotus japonicus.

Nitric oxide quantitation in legume root samples measuring the DAF-FM fluorophore. Reference: Maki Nagata, Ei-ichi Murakami, Yoshikazu Shimoda, Fuyuko Shimoda-Sasakura, Ken-ichi Kucho, Akihiro Suzuki, Mikiko Abe, Shiro Higashi, Toshiki Uchiumi Molecular Plant-Microbe Interactions 2008 21:9, 1175-1183, DOI: 10.1094/MPMI-21-9-1175


A cooperative polymer-DNA microarray approach to biomaterial investigation

Cy3 and Cy5 labeled cRNA assessed. Reference: Salvatore Pernagallo, Juan Jose Diaz-Mochon and Mark Bradley Lab Chip, 2009, 9, 397 - 403, DOI: 10.1039/b808363k


Elevated polyamines induce c-MYC overexpression by perturbing quadruplex-WC duplex equilibrium.

FRET probe binding assays. Reference: Niti Kumar, Richa Basundra, and Souvik Maiti, Nucleic Acids Res. 2009 Jun;37(10):3321-31. DOI 10.1093/nar/gkp196.


Development of a Real-time RT-PCR Assay for Detecting EGFRvIII in Gliblastoma samples

Total RNA quantitation with Quant-iT RiboGreen® assay. Reference: Koji Yoshimoto, Julie Dang, Shaojun Zhu, David Nathanson, Tiffany Huang, Rebecca Dumont, David B. Seligson, William H. Yong, Zhenggang Xiong, Nagesh Rao, Henrik Winther, Arnab Chakravarti, Darell D. Bigner, Ingo K. Mellinghoff, Steve Horvath, Webster K. Cavenee, Timothy F. Cloughesy, and Paul S. Mischel Clin Cancer Res January 15, 2008 14:488-493; doi:10.1158/1078-0432.CCR-07-1966


Lack of A1 adenosine receptors augments diabetic hyperfiltration and glomerular injury.

Measured mouse glomerular filtration rate using FITC labeled inulin. Reference: Faulhaber-Walter, Robert, Chen, Limeng, Oppermann, Mona, Kim, Soo Mi, Huang, Yuning, Hiramatsu, Noriyuki, Mizel, Diane, Kajiyama, Hiroshi, Zerfas, Patricia, Briggs, Josephine P., Kopp, Jeffrey B., Schnermann, Jurgen J Am Soc Nephrol 2008 19: 722-730, doi: 10.1681/ASN.2007060721.


Controlled drug release from multilayered phospholipids polymer hydrogel on titanium alloy

Determined the concentration of pyrene labeled phospholipid polymer released into solution. Reference: Jiyeon Choi, Tomohiro Konno, Madoka Takai and Kazuhiko Ishihara, Biomaterials Volume 30, Issue 28, October 2009, Pages 5201-5208, doi:10.1016/j.biomaterials.2009.06.003

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