Enhanced Forward Scatter (eFSC)

With the MoFlo Astrios EQ, you never have to be limited by your instrumentation. Its patent pending dual enhanced forward scatter (eFSC) technology enables you to see and sort particles you might otherwise miss. Measure crucial particle characteristics over more than three orders of magnitude, from as small as 200 nm to 30 µm diameter, all at the same time. You can also trust MoFlo’s proven fluidics to be gentle with your biological material.

• 7 lasers, 2 alignment stages
• Resolve particles (beads and cells) down to 0.2 µm
• Sort rare event populations with 99% purity
• 6-way mixed mode sorting gives you flexibility in experiment design
• Forward scatter detection support for 6 laser paths
• Optional side scatter off any laser (excluding UV)
• Minimum triggering threshold reduced to 0.001% to allow more resolution.
• Seven mask designs for differing applications
• ABS ND filter set. 0.3, 0.6, 1.0, 1.3, 2.0
• Fluidic and air filtration enhancements:

• Sheath filter 0.04 µm particles
• Air filter 0.01µm particles
• Unequalled resolving FSC & FL power through adjustable 9-decade dynamic range delivering greater certainty when resolving background from dim staining populations and/or broad dynamic range applications

Excitation Optics

Laser Palette:

Number of laser interrogation points: 7 discrete spots

Number of Pinholes: 7 Pinholes

Standard Filter Sets optimized per laser wavelength:
355nm – PI, Hoechst (red and blue), DAPI
405nm – SSC, Cascade Blue*, Pacific Blue*, Cascade Yellow*
488nm – SSC, FITC, PE, ECD (PE-Texas Red*), PE-Cy5/PerCP, PE-Cy5.5, PE-Cy7
532nm – SSC, PE, PE-Alexa* 610, PE-Alexa 647, PE-Cy5.5, PE-Alexa 700
561nm – SSC, mCherry, DSRed, mPlum, HCRed
592nm – SSC, Texas Red, APC, APC-Cy7, Alexa 647, Alexa 700, Alexa 750
642nm – SSC, APC, APC-Cy7, Alexa 647, Alexa 700, Alexa 750

Bringing new data within reach

Emission Optics

Resolution:
Resolves minimum 0.2μm diameter particles in scatter from background.
Fluorescence Sensitivity:
Fluorescence sensitivity of < 125 MESF for FITC and < 110 MESF for PE using
Spherotech 8-peak beads using 488nm laser
Optical Parameters:
Up to 44 including 2 FSC.
Fluorescence half height CV precision:
FITC <2.4%, PE <2.4%, PE-TR <2.6%, PE-Cy5 <3.2%,
PE-Cy7 <4.3%, APC <2.3%,
APC-Cy7 <2.8%

(1 x 106 SPHERO™ Ultra Rainbow beads at sample flow rate of 6 μL/min, 100-120 EPS)

IntelliSort II Beadless Drop Delay

Minimize your risk of sample contamination and maintain integrity of sorted samples with uninterrupted aseptic sorting.

• No fluorescent beads or microscope required
• Camera images and proprietary algorithms enable operator to determine optimal drop delay without the use of fluorescent particles in the sample line
• Eliminates requirement to remove sterile samples during drop-delay recalculation due to nozzle blockages, thus ensuring uninterrupted sterility
• Maintain drop delay functionality with 50µm and 150µm nozzle tips
• New Auto-Quality Control parameters: sheath pressure, sample pressure, average event rate, start and end times, nozzle size, differential between measured parameters and pass/fail thresholds, and the ability to exclude QC data
• New laser-delay parameters: date of last laser delay calculation, trigger channel, sheath pressure, nozzle size, laser wavelengths that are powered, calculated laser delays displayed

Summit v6.2 Software

Enjoy faster, easier instrument set-up and leading edge features and functionality.

• Support for multiple forward scatter parameters in Summit and FCS file format
• 2 additional PMT channels for forward scatter collection
• Minimum allowable PMT voltage changed from 400 volts to 100 volts to enhance useable dynamic range.
• Enhanced functionality and support for user-defined filters and fluorochromes.
• Enhanced automated quality control functionality

Astrios EQ brochure

View		The Evolution Continues
		Researchers rely on Beckman Coulter's historic cell sorter technology. Now the next generation is here. The MoFlo Astrios is the new standard for cell sorting with functionality, speed, and improved sensitivity. It is designed for researchers who desire high productivity with more analytical capability and with biosafety solutions.

Application Flexibility

The MoFlo Astrios EQ is designed to allow you to work with the greatest possible range of biological and non-biological particles, and you can fine-tune the instrument to suit your application. Adjust pressures to handle a wide range of cell types, improve cell vitality without compromising sort speed, and keep your cells alive and running with temperature and agitation options.

The MoFlo Astrios EQ is designed so that your application determines your instrument parameters, not the other way around. This flexibility enables you to conduct a wide range of single-cell applications, including:

• Circulating Tumor Cells (CTCs)
• Drug discovery applications
• Rare event analysis
• Stem cells
• Neuronal cells
• Fluorescent protein reporter selection
• Ecological and physiological microbes (yeasts and bacteria)

In conventional cell sorters, there is a three-way trade-off between sort speed, sort purity and sort yield. The MoFlo Astrios EQ Mixed Mode sorting allows selecting for purity in one sort-direction and yield in another, enabling simultaneous recovery of virtually all the target cells that would otherwise be lost to achieve that high purity. This enables you to sort faster without losing extremely valuable cells, and without sacrificing purity of the principal sort.

Perform 6-way sorting without compromise:

• Save time by selecting more populations at once
• Save precious sample with our unique mixed-mode sorting capability 

Break free from cuvette sorting restrictions:

• Analyze heterogeneous populations with confidence — no differential velocities based on particle size; eliminate the need for Area: Height scaling and provides laser-delay optimization
• Maintain consistent performance with less maintenance — no concern about burn-in of debris on cuvette
• Allow biological demands to dictate sorting conditions — select from 70 and 100 micron nozzle sizes, to precisely accommodate cells from practically any tissue you want using Intellisort II to automatically maintain drop delay

See more with enhanced Forward Scatter (eFSC):

Look at data differently with 2 forward scatter PMTs

Dual eFSC channels allow you to apply different application-specific masks for different particle types, or simply use different neutral density filters to allow a greater overall particle size range to be measurable simultaneously. The top histogram was measured on eFSC2 without any neutral density (ND) filter, to widen the separation between noise and 0.2 and 0.3 μm beads. The middle and lower histograms show the eFSC1 data (acquired with an ND1 filter) with different y-axis scales to better display the larger particle peaks. The entire dynamic range from 0.2 to 30 μm is easily made visible when different ND filters are used on each eFSC parameter.