Conjugation Kits

Quick & easy-to-use conjugation kits for over 45 labels.

Antibody conjugation, also known as antibody labeling, chemically links an antibody to a specific tag. When conjugated, a primary antibody directly detects the target antigen of interest, without the need for a secondary antibody. Labels can include fluorescent dyes, enzymes or proteins and your choice or combination of labels will depend on the experimental application (multiplex IHC,  flow cytometry, LFA, others), as well as your multiplex panel. Conjugated primary antibodies can directly detect the target antigen without the aid of secondary antibodies. Using directly labeled primary antibodies can save you a lot of time and simplify your experimental protocols.

Or Lightning-Link® conjugation kits are easy to use and allow you to conjugate antibodies in three simple steps to more than 45 labels including fluorescent proteins (Alexa Fluor® and DyLight Fluor® dyes), tandem dyes, enzymes, oligonucleotides, and gold nanoparticles. Use our fluorescent dye chart to choose the right dye to build your next multiplex IHC panel. To generate high-quality conjugates we recommend using Lightning-Link® conjugation kits to conjugate our range of carrier-free antibodies and antibody pairs specifically designed for antibody labeling. We offer 10,000 formulations free from glycerol, BSA and sodium azide to reduce antibody loss and maximize conjugation efficiency. If your antibody does not meet these buffer requirements, you can easily purify or concentrate using our range of purification and concentration kits.

Explore our range of fluorescent conjugates antibodies or learn how to generate reproducible conjugates with our antibody conjugation guide.

If you're interested in outsourcing antibody labeling to our team of experts, please get in touch and ask about our Customer Conjugation Services. Contact us today to discuss your needs.

Browse Abcam most popular conjugation kits:

Enzymes: HRP | Alkaline Phosphatase
Proteins: Biotin | Streptavidin
Fluorophores: AF®488 | AF®647 | R-PE | APC | FITC | Cy3® | Cy5®
Tandem dyes: PE/Cy7® | APC/Cy7® | PE/Cy5® | PerCP/Cy5.5® | PE/Cy5.5®
Oligonucleotide: Universal
Metal Isotopes: 169Tm | 141Pr | 160Gd | 175Lu | 165Ho
Gold nanoparticles: 40nm | 20 nm | 10 nm

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Purification Kits

Easily purify or concentrate antibodies from serum, tissue culture supernatant (TCS), and ascites fluid.

Antibody purification is a critical step in applications such as antibody conjugation, as commercially available antibodies often contain unwanted substances or buffer components that may interfere with downstream processes. The purification process involves capturing the antibody on agarose beads coupled to proteins A or G, removing unwanted bSearch Abcam catalogue for Conjugation Kitsuffer constituents. This leaves the antibody in a format that is easily transferred to a conjugation-friendly buffer for optimal performance in later applications.

Abcam’s antibody purification kits enable the rapid and efficient purification of antibodies from various sources, including serum, tissue culture supernatant (TCS), and ascites fluid, producing highly purified antibodies that are fully compatible with our conjugation kits. 

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Sample Preparation Kits

Sample preparation kits provide all the reagents you need to standardize and often speed up your workflow.

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Chromogen Kits

Our range of chromogens to complete your IHC experiment.

Chromogens are compounds that produce a visible color after a chemical reaction, making them essential for visualizing target antigens in immunohistochemistry (IHC). When used with enzyme-linked antibodies, chromogens allow researchers to detect specific proteins in tissue samples. Abcam offers a wide range of chromogen and substrate kits designed for reliable, high-quality staining, providing clear and consistent results to support your IHC experiments.

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 IHC Kits

More specific and sensitive staining, even with low abundance markers.

Abcam offers an unrivaled range of recombinant, IHC-validated antibodies and IHC antibody panels for key immuno-oncology targets. We also offer IHC kits, reagents, and accessories designed to help you get specific and sensitive staining even when detecting low abundance markers.

We collaborated with NanoString to develop GeoMx Protein Assays, high-plex cocktails of oligonucleotide-labeled antibodies bound to tissue sections using standard IHC staining methods. These assays are powered by our IHC-validated recombinant antibodies to ensure high-quality results you can trust. 

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CHIP Kits

Kits for a variety of samples to help you progress in ChIP.

Chromatin immunoprecipitation (ChIP) kits are kits that enable you to perform ChIP experiments quickly and easily. ChIP assays use an antibody to bind a specific protein or modified protein. In the assay, chromatin regions associated with this protein are captured for analysis, typically with qPCR or sequencing.

Our portfolio of ChIP kits includes kits for use with a wide variety of samples (eg plant tissues, FFPE, or low cell numbers) as well as high sensitivity and one-step kits - our most popular ChIP kit (ab500) has over 130 references from our customers. We also offer ChIP-grade antibodies, validated to a higher standard in ChIP-qPCR or ChIP-seq, to use with our kits to provide further opportunities for success in your downstream sequencing. For more information, including how to select the right ChIP kit for your experiment, click here.

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Részletes termékinformációt talál a gyártó weboldalán: abcam.com