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SpectraMax iD5 Multi-Mode Microplate Reader

Your All Access Pass to Unleash Your Brilliance
The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. In addition, the SpectraMax iD5 can be expanded to include bottom-read luminescence, TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection.
With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD5 is your all access pass to helping you unleash your brilliance.

ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK!

Leírás

Key Features

Easily set up protocols, run experiments, view tutorial videos, and control user access with the intuitive SoftMax Touch Software for the touchscreen
Automatically identify filter sets using NFC functionality, simplifying workflows and eliminating confusion
Perform western blot detection with your microplate reader
Push data to workstations using QuickSync, eliminating the need to retrieve data from the instrument
Detect assay signal in the best way using the superior hybrid monochromator- and filter-based optical system
Run injector assays with SmartInject™ Technology, ensuring complete reagent mixing and rapid signal development

AUTOMATIC, NFC-ENABLED FILTER IDENTIFICATION

Using built-in near-field communication (NFC) tags, the SpectraMax iD5 plate reader automatically detects the identification code of the filter to recognize the slide and filter configuration, eliminating confusion and simplifying your filter workflow.

WESTERN BLOT CAPABILITY

Protein detection is an important task for pharmaceutical and clinical research, and Western Blots are one of the most common methods employed for this purpose. The SpectraMax iD5 plate reader, utilizing an optional enhanced TRF detection module, is capable of scanning and analyzing your membranes for western blot data. The Europium-incubated membranes are resistant to photo bleaching and allow you to read the membranes without loss of signal for longer periods.

HYBRID MICROPLATE READER

Detect assay signal the best way using the hybrid monochromator- and filter-based optical system.

QUICKSYNC DATA PUSH TECHNOLOGY

Reduce the amount of time you spend collecting data and increase your productivity. The SpectraMax iD5 reader allows you to interact with your data any way you want from anywhere you want. View your data quickly using the large touchscreen interface, export your data to a USB drive for analysis in the program of your choice, or analyze your data using the industry’s leading data acquisition and analysis tool, SoftMax Pro 7 Software. The SpectraMax iD5 reader also features network connectivity that allows you to walk away from the instrument to focus on additional research. Data is automatically delivered to any workstation on the same network, eliminating the need to physically retrieve data from the instrument.

NFC TOUCHSCREEN

The innovative SpectraMax iD5 is the first five mode microplate reader featuring near-field communication (NFC) technology for touchscreen personalization, user acess control, and filter identification.

FLEXIBLE TEMPERATURE CONTROL

Simple-to-use temperature control allows you to adjust your experiment’s conditions from ambient up to 66°C, expanding your laboratory’s capabilities to include temperature sensitive assays.

DUAL AUTO INJECTORS

The SpectraMax iD5 plate reader is injector-ready allowing you to expand your lab’s capabilities to include flash applications such as dual luciferase and ATP assays. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 μL), overflow protection, and ensures equal mixing across the plate for high-precision experiments.

Műszaki specifikációk

General specifications	SpectraMax iD3	SpectraMax iD5
Read modes	Absorbance Fluorescence (top/bottom read) Luminescence (top read)	Absorbance Fluorescence (top/bottom read) Luminescence (top/bottom read) Time-Resolved Fluorescence (top/bottom read) Fluorescence Polarization (top/bottom read) HTRF (top/bottom read) HTRF
Wavelength ranges	Abs: 230 - 1000 nm FL Ex: 250 - 830 nm FL Em: 270 - 850 nm Lumi: 300 - 850 nm	Abs: 230 - 1000 nm FL Ex: 250 - 830 nm FL Em: 270 - 850 nm Lumi: 300 - 850 nm TRF Ex: 350 nm TRF Em: 450 - 750 nm FP: 300 - 750 nm
Wavelength selection	Monochromator tunable in 1-nm increments	Hybrid filters and monochromator tunable in 1 nm increments
Absorbance photometric accuracy/linearity	< ±0.010 OD ±1.0%, 0 - 3 OD VIS 0 - 3 OD UV	< ±0.010 OD ±1.0%, 0 - 3 OD VIS 0 - 3 OD UV
Absorbance photometric precision/repeatability	< ±0.003 OD ±1.0%, 0 - 3 OD VIS 0 - 3 OD UV	< ±0.003 OD ±1.0%, 0 - 3 OD VIS 0 - 3 OD UV
Sensitivity top read optimized	FL: 1 pM fluoresein 96 well, 1 pM fluorescein 384 well Lumi: 2 pM ATP 96 well, 4 pM	FL: Mono: 1 pM fluorescein 96 well, 1 pM fluorescein 384 well; Filters: 0.3 pM fluoresein 96 well, 0.5 pM fluorescein 384 well Lumi: 2 pM ATP 96 well, 4 pM ATP 384 well TRF (filters with enhanced TRF module): 10 fM Europium (2 amol/well) 96 well, 10 fM Europium (1 amol/well) 384 well FP: Mono: 2 mP @ 10nM 96 well, 2 mP @ 10nM 384 well; Filters: 1 mP @ 1 nM 96 well, 2 mP @ 1 nM 384 well
Light source(s)	High power xenon flash lamp	High power xenon flash lamp
Detector(s)	Silicon photodiode Ultra-cooled photomultiplier tube	Silicon photodiode Ultra-cooled photomultiplier tube
Read types (endpoint, kinetic, etc)	Endpoint Kinetic Spectrum scan Well scan	Endpoint Kinetic Spectrum scan Well scan
Read times (kinetic interval for 96-well plate)*	Abs: 28 seconds FL: 21 seconds Lumi: 21 seconds	Abs: 28 seconds FL: 21 seconds Lumi: 21 seconds TRF: 25 seconds FP: 35 seconds
Plate shaking	Linear Orbital Double orbital	Linear Orbital Double orbital
Injectors	dual injector, optional	dual injector, optional
Western blot	not available	with optional cartridge
Stand-alone touchscreen operation	yes	yes
Robotics / automation	compatible	compatible
Plate type(s)	6-384 well plates	6-384 well plates
Temperature control	Ambient + 5°C to 66°C	Ambient + 5°C to 66°C
Dimensions	H 40.1 cm x W 53.2 cm x D 59.8 cm	H 40.1 cm x W 53.2 cm x D 59.8 cm
Validation tools	Absorbance validation plate Fluorescence validation plate Luminescence validation plate SoftMax® Pro GxP Software	Absorbance validation plate Fluorescence validation plate Luminescence validation plate Multi-mode validation plate SoftMax® Pro GxP Software

Applikációk

The SpectraMax iD5 is a hybrid monochromator- and filter-based, five-mode microplate reader that allows you to expand the boundaries of your research to include a wide variety of applications.

Cell biology
Protein biology
Apoptosis
BRET
Cellular imaging assays
Cell cycle analysis
Cell proliferation, viability, cytotoxicity
Enzyme activity
Epigenetics
Flash based applications including dual luciferase and ATP assays
Fluorescent protein expression
FRET
Genotyping
GPCRs
HTRF®
Immunoassays
Ion channels
Membrane potential assays
Micro Volume (Low volume) Applications
Molecular interactions
Nuclear receptor
Nucleic acid and Protein quantitation
Reporter assay
Time-Resolved Fluorescence (TRF)
Western blot detection
Cell line development
Antibody discovery
Screening
Genomics

Videók, ismertetők

Five-Mode Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability

SpectraMax iD5 Multi-Mode Microplate Reader Brochure

Five-mode microplate reader with automatic filter identification and western blot capability The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. In addition, the SpectraMax iD5 reader can be expanded to include bottom-read luminescence, TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD5 reader is your all access pass to helping you unleash your brilliance.

Click here to download the product brochure >>

Application Note: Detect inflammatory cytokines at picogram levels with Sword ELISA Boosters

Traditional ELISA methods using the horseradish peroxidase (HRP) substrate 3,3’,5,5’- tetramethylbenzidine (TMB) often fail to detect low abundance analytes, such as inflammatory cytokines. The Sword ELISA Booster, a next-generation ELISA detection technology that directly replaces the detection reagents in a traditional, gold-standard ELISA, increases sensitivity up to 30 fold.

This enhanced assay allows you to detect lower levels of these cytokines and advance your understanding of diseases associated with these mediators of inflammation. Here, we demonstrate how three SpectraMax® microplate readers we tested met the criteria for sensitivity outlined for the Sword ELISA Boosters.

Click here to download the application note >>

Application Note: Monitor NF-κB activation with a sensitive dual reporter assay on the SpectraMax iD5 microplate reader

SpectraMax iD5 Application Note - Monitor NF-κB activation with sensitive dual reporter assay

Reporter genes are valuable tools for studying gene expression, serving as surrogates for genes involved in various signaling pathways and disease conditions. Luciferases are the most common reporter genes, as they are easily detectable using luminometers or luminescence microplate readers, and the low luminescent background present in cells enables high assay sensitivity. Firefly luciferase is typically used as a readout for expression of a gene of interest. Luciferase from the sea pansy Renilla reniformis is often used in multiplexed luciferase assays as a second reporter to normalize for transfection efficiency, cell viability, and other factors that may vary among assay samples. Here we show how the DuoLuc reporter assay and SpectraMax iD5 reader are used to detect nuclear factor-κB (NF-κB) activation in a mammalian cell model. NF-κB is a ‘master regulator’ of gene expression for many cellular processes including inflammation, immunity, differentiation, proliferation, and apoptosis.

Click here to download the application note >>

View SpectraMax iD3 Tutorial Videos

Getting to know your new SpectraMax iD5 Multi-Mode Microplate Reader

View a brief overview of the features and benefits of your new SpectraMax iD5 Multi-Mode Microplate Reader.
Click here to view >>

Personalizing your new SpectraMax iD5 Multi-Mode Microplate Reader

Learn how to personalize your SpectraMax iD5 Multi-Mode Microplate Reader. Create user profiles and pair them to NFC tags to easily pull up saved protocols, and experiment results.
Click here to view >>