The unit can also be used for routine operations of cells washing from culture medium and resuspension in a buffer.
The operation principle is based on creating negative pressure in the trapping flask using an built-in micro-compressor. The collecting tip is connected with polyethylene tube to the trapping flask. Liquid is removed from the microtest tube when the collecting tip touches the solution surface.
A tube holder-organiser is conveniently located on the right hand side of the FTA-1. This can accommodate two tubes e.g. one for a hydrochloric acid solution and one for a distillate which is necessary for tip washing and storing, such that a tip can be re-used.
A suction microbiological filter eliminates risk of contamination from the trap flask with bacteria, viruses and infected particles. The suction microbiological filter is hydrophobic: with efficiency up to 99.9% it holds particles bigger than 0.027 micron, which are smaller than agents of Hepatitis A, B and C.
The use of an aspirator is preferential to using a generic laboratory vacuum line as it restricts the possibility of contamination and can be used exactly where the operator is located i.e. no need for a dedicated position in the laboratory to perform the aspirator.
Aspirator - models and specifications | ||
---|---|---|
FTA-1 | ||
Aspirator with trap flask | ||
Vacuum | mbar | -500 |
Trap Flask volume | L | 1 |
Input current | V dc | 12 |
Power supply | V | 120/230 |
Flow rate (aqueous solution) | ml/min |
72 with aspiration tip 666 without aspiration tip |
Aspiration/removal of alcohol/buffer remaining quantities from microtest tube walls during DNA/RNA purification and other macromolecule reprecipitation techniques. For routine operations of cells washing from culture medium and resuspension in buffer.
EASIH Eastern Sequence and Informatics
Hub Ms Anna Livings - Senior Lab Technician
8/11/2012
Organisation Background
Advances in DNA sequencing have meant a dramatic fall in the cost and time needed for the generation of high throughput sequencing (HTS) data sets. The creation of the Eastern Sequence and Informatics Hub (EASIH) enables researchers to utilise Next Generation Sequencing, a crucial 21st-century research approach in their studies. EASIH also provides researchers with access to the tools and protocols required to analyse these complicated and large-scale datasets, and in the design and implementation of projects.
EASIH, based in Cambridge, also applies HTS to routine medical diagnostic uses, in particular in HLA typing in transplantation and cord blood stem cells, and re-sequencing of disease genes in collaboration with East Anglian Medical Genetics Service.
Grant Instruments
Anna Livings, senior lab technician at EASIH had a need to be able to aspirate and contain small amounts of liquid from the Caliper LabChip® used in the laboratory. Previously, the lab in-line vacuum system has been used; this is no longer desirable due to environmental concerns and restrictions on locations of the vacuum taps.
Using the new FTA-1 personal aspirator from Grant has solved several problems at once. An all-in-one system with pump and trap flask allows containment of the aspirant safely as well as the ability to aspirate wherever it is needed.
Aspirator - Accessories | ||
---|---|---|
= optional | Aspirator | |
FTA-1 | ||
with trap flask | ||
MA-8 optional 8 channel adaptor kit , includes tube adaptor, 8 channel aspiration tip, FTA-1 holder (image in gallery section) | ||
MA-8T replacement 8 channel tip | ||
FA-1 replacement hydrophobic filter | ||
FTA-B replacement 1L bottle, blue cap, insert and sealer | ||
FTA-T tubing set (all tubes with fittings except filter and aspiration tip) |
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