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Gemini EM Microplate Reader

Create custom fluorescence assays without having to purchase expensive filter sets. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets you determine the optimal excitation and emission settings for your creative new fluorescence intensity assays. If the best wavelengths are unknown, the Gemini EM reader will find them for you. Switch easily between top and bottom reading modes for solution- and cell-based assays.

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Leírás

Benefits

Read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes
Eliminate the need for identifying, purchasing, and changing out filters for wavelength optimization with dual monochromators for excitation and emission wavelength selection between 250 and 850 nm
Measure fluorescence from solutions or cell-based assays with the best optical sensitivity by switching between top and bottom fluorescence reading
Up to 4 wavelength pairs can be read for endpoint and kinetic measurements
Well scanning to report a fluorescent measurement from a single point in the center of a microplate well to multiple points across a tissue culture well

Measuring Accurately and Reproducibly

Avoid losing high signals to detector saturation and photobleaching after repeated reads to find the correct reader settings with AutoPMT System electronics
Compare relative fluorescence units (RFUs) between samples, like with no other fluorometer, allowed by a unique calibration against an internal standard
Monitoring of temperature-sensitive reactions with consistent temperature regulation from ambient to 45°C
Thorough mixing of samples in microplate wells by user-programmable Automix feature
Gentle movement of cell or other aggregates in the microplate with a slow carriage speed option
Seamless automation coupling with the StakMax Stacker, Molecular Devices own integrated microplate stacker designed exclusively for the SpectraMax Reader

Comprehensive Reader Validation

Molecular Devices microplate readers have the most variety of tools available for users to meet their diverse hardware and software validation needs. Molecular Devices provides complete solutions with:

SpectraTest ABS1, FL1, and LM1 Validation Plates for hardware validation of absorbance, fluorescence, and luminescence modes
SoftMax Pro Software Validation Package
Software tools for FDA 21 CFR Part 11 compliance

SoftMaxPro Software, Molecular Devices' industry leading all-in-one data acquisition and analysis software, offers:

The most extensive library of supported application protocols to get you started faster
Optional tools for regulated labs with SoftMax Pro GxP Compliance Software
Advanced calculations including non-linear fitting metrics for in-depth analysis
The only Mac- and Windows-native application support for seamless exchange of data Files between operating systems
Proven integrations with leading automation and LIMS platforms for partnered solutions

Műszaki specifikációk

General Specifications
Dimensions (in)	8.6 H x 22.8 W x 15 D
Dimensions (cm)	22 H x 58 W x 38 D
Weight	35 lbs (16 kg)
Power Source	100-240 VAC, 50/60 Hz
Performance Specifications
Wavelength Range-Excitation	250-850 nm
Wavelength Range-Emission	250-850 nm
Wavelength Selections	Monochromator, tunable 1.0 nm increments
Wavelength Bandwidth (Excitation and emission)	9nm
Wavelength Accuracy	< ± 2.0 nm
Top Read Sensitivity	3.0 fmol/well FITC 200 µl in 96 wells
Bottom Read Sensitivity	8.0 fmol/well FITC 200 µl in 96 wells
Light Source	Xenon flash lamp
Microplate Read Time
Normal Mode	96 wells	15 seconds
	384 wells	45 seconds
Dynamic Range	6 orders of magnitude
Temperature Range	Ambient + 4°C up to 45°C
Temperature Unitformity (Microplate)	± 1°C @ 37°C

Applikációk

The Gemini EM Microplate Reader supports a multitude of fluorescence assay categories including:

ELISAs and immunoassays
Nucleic acid (DNA) quantitation
Protein quantitation
Reporter gene assays
Cell Viability, proliferation, and cytotoxicity
Enzyme assays
Transporter assays
Phosphotases/kinases
Microbial growth

Tartozékok, kiszerelések

Product	Part Number
Gemini EM Microplate Reader	EM
SpectraTest FL1 Fluorescence Validation Plate	0200-5060
SpectraDrop™ Micro-Volume Microplate Starter Kit	0200-6262
SpectraDrop™ Micro-Volume Microplate HTS Kit	0200-6267
Microplate Reader Shelf	9000-0756

Videók, ismertetők

Brochures Gemini EM Microplate Reader

Applications

Measurement of Proteasome Inhibition in Live Cells inMolecular Devices Microplate Fluorometers
Optimizing Excitiation and Emission Wavelengths for Narrow Stokes Shift Fluorophores Using the GEMINI and SoftMax Pro Software
Selecting Excitation and Emission Wavelengths Using the GEMINI Microplate Spectrofluorometer - Basic Principles
Measuring cell proliferation using the CyQUANT Cell Proliferation Assay with SpectraMax Microplate Readers

Number of Citations*: 1,340

[HTML] Paeonolum protects against MPP+-induced neurotoxicity in zebrafish and PC12 cells

XL Lu, YH Lin, Q Wu, FJ Su, CH Ye, L Shi… - BMC complementary …, 2015 - biomedcentral.com

... Quantitative measurements of fluorescence readings were determined at 485/538
nm (excitation/emission) using a SpectraMax GEMINI EM fluorescent plate reader
(Molecular Devices, USA) from cells in 96-well plates. The ...

Abnormalities in the Tricarboxylic Acid Cycle in Huntington Disease and in a Huntington Disease Mouse Model

NN Naseri, H Xu, J Bonica, JPG Vonsattel… - … of Neuropathology & …, 2015 - journals.lww.com

Close Window. Close Window. Enter your Email address: Wolters Kluwer Health may email
you for journal alerts and information, but is committed to maintaining your privacy and will
not share your personal information without your express consent. ...

Oxidative stress involving changes in Nrf2 and ER stress in early stages of Alzheimer's disease

SI Mota, RO Costa, IL Ferreira, I Santana… - … et Biophysica Acta (BBA …, 2015 - Elsevier

Oxidative stress and endoplasmic reticulum (ER) stress have been associated with Alzheimer's
disease (AD) progression. In this study we analyzed whether oxidati.