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Epigenetika

Promega is also setting the standard to develop products for epigenetic studies with a variety of products like cell-based and biochemical assays, DNA methylation kits and products for protein analysis and complex purification

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Cell-Based and Biochemical Assays

Simple luminescence-based assays for cell viability, histone deacetylase (HDAC) and sirtuin (SIRT) activities, deubiquitinating (DUB), deSUMOylating (SENP) and deneddylating (NEDP) proteases.

NanoBRET™ Protein:Protein Interaction Assays

Live-cell protein interaction assays based on Bioluminescent Resonance Energy Transfer (BRET) for protein targets in bromodomain, transcription, and other protein families. Choose from numerous prebuilt assays, or purchase vectors and reagents to build your own assays in convenient kit format.

HDAC-Glo™ I/II Assays

Measure the relative activity of HDAC class I and II enzymes from cell extracts or purified enzyme sources with these assays and screening systems.

HDAC-Glo™ 2 Assay

Selectively measure HDAC isozyme 2 activity or inhibition from cell extracts (cell-based assays) or purified enzyme sources (biochemical assays).

HDAC-Glo™ Class IIa Assay

Selectively measure HDAC class IIa activity or inhibition from cell extracts (cell-based assays) or purified enzyme sources (biochemical assays).

SIRT-Glo™ Assays

Single-reagent-addition, homogeneous, luminescent assays and screening systems that measure the relative activity of the NAD+-dependent histone deacetylase class III enzymes (sirtuins; SIRTs) from purified enzyme sources.

DNA Methylation Analysis

Kits for use in epigenetic studies evaluating DNA methylation and gene expression. Includes DNA purification, methylation-sensitive restriction enzymes, PCR kits, reporter assays and transfection reagents.

Bisulfite Conversion

The new MethylEdge™ Bisulfite Conversion System provides an efficient method to rapidly perform bisulfite conversion, giving you more intact, converted DNA for downstream applications.

PCR, qPCR and RT-qPCR

Improve PCR performance in techniques such as bisulfite-specific PCR with the GoTaq® polymerase family of master mixes.

Methylation-Specific Restriction Enzymes

We offer high-quality, dependable enzymes for cloning and DNA analysis. Promega methylation-specific restriction enzymes include: HpaII, MboI, MspI, and Sau3AI.

Luciferase-Based Methylation Detection

We offer FuGENE® HD transfection reagent and optimized luciferase genes and vectors designed for use in reporter assays. Use these tools to measure methylation status and methylation regulators precisely and with high sensitivity.

Protein Analysis and Complex Purification

Purify epigenetic proteins and epigenetic complexes, identify protein:protein or protein:DNA interactions, and study histone marks rapidly and efficiently.