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Promega

RNS-analízis

From Northern blots and RNase protection assays to cloning vectors, Promega provides flexible options to meet your gene expression analysis needs.
Suppress protein expression transiently or permanently using tools designed for producing high efficiency RNAi responses.
Convenient kits for isolating total RNA and mRNA from many sample types including blood, cells, FFPE tissue and plants. Miniprep, midiprep and automation-friendly formats.
Easy, dependable and robust reverse transcriptases and RT-PCR kits for reverse transcription of full-length cDNA from abundant and rare mRNAs.

ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK!

In Vitro Transcription

RNasin® Ribonuclease Inhibitors

Protect your RNA and avoid costly, disappointing results. Use high affinity RNAsin® Inhibitors for nearly instantaneous inhibition of a broad spectrum of RNases over a wide pH range.

pGEM®-T Easy Vector Systems

Convenient PCR cloning with the added convenience of restriction enzyme recognition sites flanking the insertion site, creating more options for removal of the insert DNA with a single digest. It's Easy.

RiboMax™ Large Scale RNA Production Systems

Consistently produce 2–5mg/ml of RNA in a 1ml reaction. Retrieve 10- to 20-fold more RNA than with the standard Riboprobe® System transcription reaction.

Riboprobe® In Vitro Transcription Combination Systems

Find complete in vitro transcription systems that include RNA polymerases, reagents (excluding radioisotope) and RQ1 RNase-Free DNase (Cat.# M6101) for DNA template removal after transcription.

RNA Interference

psiCHECK™ Vectors

Rapidly screen RNAi by quantifying the Renillaluciferase reporter gene activity. Avoid labor-intensive, time-consuming assays and waiting for phenotypic changes.

T7 RiboMAX™ Express RNAi System

Generate milligram amounts of protein-free dsRNA in less than 30 minutes. The dsRNA is suitable for RNAi in both mammalian and nonmammalian systems.

pmirGLO Dual-Luciferase miRNA Target Expression Vector

Quantitatively measure microRNA activity with a luciferase reporter. The firefly luciferase (luc2) vector is the primary reporter to monitor mRNA regulation and Renilla luciferase (hRluc-neo) acts as a control reporter for normalization and selection.

 

RNA Purification

simplyRNA for Maxwell® RSC Instruments

The Maxwell® RSC simplyRNA Kits allow easy, automated RNA purification from cells or tissues, providing consistent yields of high-quality RNA for use in downstream applications.

Maxwell® RSC RNA FFPE Kit

Use this system with the Maxwell® RSC Instrument to rapidly extract RNA from FFPE tissue. High yields of concentrated RNA. No need to concentrate eluates before RT-PCR.

Maxwell® 16 LEV Plant RNA Kit

Extract RNA from challenging plant samples. Process 1-16 samples in 60 minutes with minimal preprocessing, increasing consistency in RNA purification from soybean, corn andArabidopsis samples.

ReliaPrep™ RNA Cell and Tissue Miniprep Systems

A 30-minute protocol for extracting high-quality, concentrated RNA from 1 × 102–1 × 106 cultured cells or 0.25–20mg tissues.

ReliaPrep™ FFPE RNA Miniprep System

Isolate RNA from FFPE tissue in 1.5 hours with the ReliaPrep™ FFPE RNA Miniprep System. No need for hazardous solvents or overnight digestion!

SV Total RNA Isolation

This system provides a fast, miniprep-scale RNA isolation system using spin or vacuum protocols. A 96-well format is available for high-throughput manual processing and has been automated on several liquid handlers.

 

RT-PCR

GoScript™ Reverse Transcription System

Produce multiple cDNAs using oligo(dT)15 or random primers during first-strand cDNA synthesis and/or divide into multiple second-strand cDNA syntheses and PCR amplifications with different gene-specific primers.

M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant

A cost-effective, quality reverse transcriptase for routine RT-PCR and cDNA synthesis.

AccessQuick™ RT-PCR System

Saves you four pipetting steps by combining several components into a master mix.

AMV Reverse Transcriptase

Great for RT-PCR templates with high secondary structure.

 

www.promega.com 

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