digitalMLPA: Where MLPA Meets NGS for Broad Copy Number Certainty

Robust

Uniform coverage for accurate CNV results.

Reliable

High gene coverage for comprehensive CNV detection.

Simple

Six easy steps with minimal hands on time and direct-to-sequencer loading capability. 

Applications

Our current panel range focuses on hereditary cancer markers, as well as hematological malignancies, including multiple myeloma and acute lymphoblastic leukemia. In addition to our current products, we are actively developing new digitalMLPA assays for a wide range of applications.

How does digitalMLPA work?

digitalMLPA is a PCR-based technique that enables the detection of up to 1200 targeted DNA sequences in a single multiplex reaction. After the PCR-based amplicon generation, each probe amplicon is quantified using an Illumina NGS platform. digitalMLPA does not require post-PCR clean up or library concentration measurements, making the technique efficient and user-friendly.

Coffalyser digitalMLPA

Coffalyser digitalMLPA™ is our free and easy-to-use data analysis software that makes analysing digitalMLPA experiments fast and effortless. Coffalyser digitalMLPA can automatically recognise digitalMLPA sequence reads from FASTQ files, and directly uses them for data analysis. Thanks to its advanced quality checks and algorithms, Coffalyser digitalMLPA can quickly detect and analyse aberrations in your samples, providing you with clear and detailed reports for every experiment.

SALSA® digitalMLPA™ products are for research use only (RUO). Not for use in diagnostic procedures.

Learn more: https://www.mrcholland.com/technology/digitalmlpa

General information: https://www.mrcholland.com/