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SALSA® MLPA |
MLPA is the gold standard for the quantification of copy numbers. Up to 60 different genomic DNA sequences can be quantified simultaneously. MLPA products are used worldwide to study hereditary disorders, tumour profiling and methylation status in diagnostic and research.
ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK!
MLPA® (Multiplex Ligation-dependent Probe Amplification) is the gold standard for DNA copy number quantification and is used to study both hereditary disorders and tumours. MLPA can also be used to determine if a known mutation is present in the DNA or to investigate the methylation status of DNA sequences. MRC-Holland, the inventor and exclusive manufacturer of MLPA assays, offers more than 400 different MLPA panels used for DNA copy number quantification, mutation identification, and DNA methylation status.
Popular MLPA applications
- Predisposition to Cancer
- BRCA1, BRCA2, MLH1, MSH2, MSH6, PMS2, APC, MMR, NF1
- Neuromuscular Disorders
- Intellectual Disability
- Solid Tumours
- Methylation profiling
- Prader-Willi / Angelman syndrome, Fragile X, Beckwith-Wiedemann Syndrome
Advantages and features of MLPA
Examine up to 60 targets per reaction
Only 50 ng of DNA is required per reaction
MLPA detects a short sequence of 60-80 nucleotides
Detection of single exon deletions and duplications
Detection of targeted point mutations
One simple protocol
Results available within 24 hours
Limited lab equipment required: only a thermocycler and a sequence type electrophoresis system
User-friendly, reliable, and free data analysis software: Coffalyser.Net
Visit the website mrcholland.com to:
- Search for genes, and chromosomal regions of interest to find all related MLPA products.
- Explore the MLPA procedure to learn how to do MLPA
- Get MLPA support by viewing education, workshop, and troubleshooting materials.
- Stay up to date with current MLPA news.
Videók, ismertetők
MLPA Essential Points (pdf)
MLPA® General Protocol (pdf)
MS-MLPA® General Protocol (pdf)
The principle of MLPA is explained in the video below. Read on for a textual explanation.
Tartozékok, kiszerelések
Coffalyser.Net: MLPA analysis software
Coffalyser.Net™ is free, user-friendly MLPA analysis software developed and supported by MRC Holland to help you get the most out of your data.
Advanced quality control
An advanced system of quality checks designed specifically for MLPA helps you evaluate everything from the quality of the fragment separation and the MLPA reaction to the reproducibility of your reference samples. These checks help prevent you from inadvertently drawing conclusions based on poor data, and boosts your confidence in the reliability of your results.
Visualise all aspects of your data
Coffalyser.Net allows you to visualise and analyse your data in multiple ways. You can look at electropherograms, tables, or focus on charts with copy number ratios. For more complex cases and troubleshooting, you can view the results of intermediate analysis steps. To discuss or store your results, you can export a summary as PDF.
Access the latest information
The latest information about our products can be retrieved directly from our servers from within Coffalyser.Net. This ensures that you can start analyzing right after receiving a new product. Updates to Coffalyser.Net are, and always will be, free, ensuring that you always profit from the latest insights.
Free support
We fully support Coffalyser.Net. We are always happy to answer your questions on how to use the software, and to help you troubleshoot difficult data.
To get started with Coffalyser.Net, visit:
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