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Molecular Devices

Axopatch 200B Amplifier, Patch Clamp, Elektrofiziológia
200b
axopatch_200b_270
For single-channel recording, whole-cell voltage- and current-clamp recordings, extracellular field potential recordings in ion channel, neuroscience, nanopore, voltammetry studies

The benchmark for single-channel recordings and other applications where an excellent signal-to-noise ratio is key.

ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK!

Widely accepted as the gold-standard for ultra low-noise patch clamp recordings, the Axopatch™ 200B Capacitor Feedback Patch Clamp Amplifier is the premier microelectrode amplifier for ultra-low noise patch clamp recordings. Incorporates capacitor-feedback technology and active headstage cooling for single-channel recording, while resistive-feedback circuitry is used for two different ranges of whole-cell recording.
 
In current-clamp mode, offers two different speeds to optimize membrane potential recording, and allows correction for voltage errors due to pipette resistance. This makes the Axopatch 200B System the amplifier of choice for a scientist who performs basic research in the ion channel field and is the reason why researchers purchased thousands of these amplifiers from Molecular Devices over the past decade.
 
  • Actively cooled capacitor-feedback headstage reduces thermal noise for signal-to-noise ratios close to the theoretical limits of physics
  • Integrating headstage mode for measuring sub-picoamp currents to enable high-fidelity single-channel recordings from cell membranes and artificial bilayers
  • Resistive headstage mode for larger currents and noise levels in whole-cell recordings
  • Slim headstage design for easier access to the preparation on a crowded microscope stage
  • Series resistance compensation: Prediction helps achieve the desired command level faster by transiently supercharging the membrane potential
  • Series resistance compensation: Correction improves the bandwidth of the recording by eliminating the error introduced by the voltage drop across the series resistance
  • Dual-speed current clamp optimizes speed and stability through better matching of the clamp algorithm to experimental parameters
  • Integrated seal test in voltage- and current-clamp mode for easy monitoring of membrane and cell health parameters
  • Dual command potentials add flexibility by processing input signals from two different sources
  • Selectable holding command input with two internal range settings and an external input enable convenient control of the output waveform
  • Pipette capacitance compensation neutralizes the contribution of the glass pipette and the pipette holder to the capacitance of the circuitry
  • Cell capacitance compensation corrects for the capacitance of the cell membrane in whole-cell recordings
  • Selectable output gain with 10 settings to scale the output signal to the desired level
  • Leak subtraction for correction of leak currents without software-based algorithms
  • Sophisticated 4-pole Bessel low-pass filter acts as anti-aliasing filter and can be used to pre-condition the output signal
  • Configurable panel meter displays user-selectable parameters in real time for quick monitoring of the experiment
  • Zap facilitates patch rupturing by applying a voltage pulse for a user-defined duration
  • Two included pipette holders of different barrel lengths accept glass pipettes in most standard dimensions
  • User guide written by scientific consultants, with the assistance of Axon Instruments staff, for straightforward start-up and as an in-depth reference

Műszaki specifikációk
General Specifications
Dimensions (in.) 3.5 (H) x 19 (W) x 12.5 (D)
Dimensions (cm) 8.9 (H) x 48.3 (W) x 31.7 (D)
Weight 11.5 (5.1 kg)
Headstage (in.) 0.75 (H) x 0.70 (W) x 4.2 (D)
Headstage (cm) 1.8 (H) x 1.9 (W) x 10.5 (D)
Mounting plate (in.) 0.25 (H) x 2.0 (W) x 2.5 (D)
Mounting plate (cm) 0.6 (H) x 5.0 (W) x 6.2 (D)
Communications Analog and digital BNC
Rack use Standard 19" rack-mount (2U) with handles
Benchtop use Bayonet feet
Power
85-264 VAC (110-340 VDC)
50-60 Hz, 30 W (max)
Fuse 0.5 A slow (15 x 20 mm)
Line filter RFI filter included
Line cord Shielded line cord provided
Safety CE marking (Conformité Européenne)
Applikációk

Basic Ion Channel Research

Ion channels are pore proteins that are involved in a large variety of physiological processes. Basic research in the ion channel field furthers the understanding of ion channel function as well as their roles within cellular pathways and processes. Single-channel research helps with elucidating the mechanisms underlying ion channel function on a single-molecule level. Whole-cell and perforated patch clamp recordings are typically used to investigate the action of modulators on entire populations of ion channels in the membrane of a cell. 

Cell Pathway Analysis

Ion channels are involved in many cell pathways and understanding the function of ion channels in response to changes in membrane potential or the presence or absence of other molecules is important in order to understand exactly how ion channels participate in normal and abnormal biological processes such as cell differentiation and migration, disease states, neuronal communications, etc. 

Disease Research

Ion channels play a role in many diseases, including hypertension, cardiac arrhythmias, gastrointestinal, immune and neuromuscular disorders, pathological pain and cancer. By understanding the exact role that ion channels play in a particular disease, researchers might be able to find a way to affect the ion channel in such a way as to alter the course of the disease.

Safety Assessment

A critical part of drug discovery involves safety assessments, such as hERG testing, in order to eliminate any compounds with potential safety concerns. Conventional patch clamp has played an important role in collecting evidence for the absence of side effects before submission of a drug candidate to regulatory authorities. Even if automated electrophysiology has shifted safety testing to earlier stages in the drug discovery process, follow-up with conventional patch clamp is an essential step in the workflow. 

Tartozékok, kiszerelések
CV 203BU Headstage
 
Construction:
 All critical components are in a sealed hybrid and cooled with a solid state cooling element
Configuration: High-speed, low-noise current to-voltage converter
Cooling: Input circuitry -15°C typical. Headstage cooling should be kept on at all times to ensure proper calibration of offset voltages
Gain (b):
1 mV/pA (b = 1) Patch or Whole-Cell modes
0.1 mV/pA (b = 0.1) Whole-Cell mode
Feedback Element:
Patch 1pF
Whole Cell β = 1, 500 MΩ in parallel with 1 pF
Whole Cell β = 0.1, 50 MΩ in parallel with 1 pF
 
 
 
 
Tuning (Whole Cell mode only): Tuning circuit to idealize response of the feedback resistor is contained in the main instrument. Tuning is automatically bypassed when the capacitive feedback is selected.
 
Pipette-capacitance-compensation injection capacitor: 1 pF
 
Whole-Cell-Capacitance-Compensation Ranges:
Patch none
Whole Cell β = 1 : 0.3-100 pF
Whole Cell β = 0.1 : 3-1000 pF
  • Case: Connected to ground. Case jack mates to 1 mm plugs.
  • Bandwidth: Test signal applied via Speed Test input
    • Internal:
      • (Patch mode): Internal: 140 kHz
      • 70 kHz whole-cell mode
    • External:
      • 100 kHz (limited by Bessel filter)
  • Capacitative load stability: 1000 pF, 0 Ω in series
  • Maximum instrument noise: Measured with minimal external noise sources ( i.e. radiated line-frequency noise, mechanical vibration) 8-pole Bessel filter.
Max. Instrument Noise: With 1-HL-U Holder
  Patch Whole Cell Whole Cell
Line Frequency and Harmonic β = 1
0.005 pAp-p		 β = 1
0.005 pAp-p		 β = 0.1
0.005 pAp-p
0.1 - 100 Hz 0.030 pAp-p 0.50 pAp-p 1.6 pAp-p
0.1 - 1 kHz 0.015 pArms 0.50 pArms 0.75 pArms
0.1 - 5 kHz 0.060 pArms 0.65 pArms 1.65 pArms
0.1 - 10 kHz 0.130 pArms 1.10 pArms 3.0 pArms
Max. Instrument Noise: Without Holder
  Patch Whole Cell Whole Cell
Line Frequency and Harmonic β = 1
0.005 pAp-p		 β = 1
0.005 pAp-p		 β = 0.1
0.005 pAp-p
0.1 - 10 kHz 0.145 pArms 1.10 pArms 3.0 pArms

In combination with a Digidata® 1550 Data Acquisition System and pCLAMP™ Software, the Axopatch 200B Amplifier forms the foundation of a patch clamp setup like those used in many electrophysiology labs around the world. Telegraphing between the software and the amplifier ensure correct scaling and logging of experimental parameters. The Clampex Data Acquisition Module, which is part of the pCLAMP Software, makes optimal use of the many advanced features of the Axopatch 200B Amplifier.

 
Videók, ismertetők

Axopatch 200B ismertető

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