ScanLater Western Blot Detection Cartridge

SpectraMaxi3-MiniMax300_carttray-WB_web_image_0

Transform your SpectraMax® i3 or Paradigm® System into a western blot readerThere are often a great many western blots along the path between imagination and understanding. Now you can read them using the advanced optical technology that's already in your lab — inside your microplate reader. The ScanLater™ Western Blot Detection System includes the ScanLater Western Blot Detection Cartridge, ScanLater Western Blot Assay Kit, and image acquisition powered by SoftMax® Pro Software. In just minutes, you can install the ScanLater Western Blot Detection Cartridge in a SpectraMax® i3 or SpectraMax® Paradigm® System to add western blot detection capability, rather than buying a dedicated western blot detection system. See more, and do more, with your microplate reader.

ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK!

Leírás

The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3 and SpectraMax Paradigm Multi-Mode Platforms. Leveraging this novel technology, researchers can:

Add western blot detection to their microplate reader within 2 minutes
Eliminate time-dependent substrate addition steps
Maintain femtogram to picogram protein sensitivity comparable to traditional western blot methods
Sustain blot signal stability for up to 30 days
Use a single software platform SoftMax Pro Software, to run both microplate and western blot detection assays

Features:

User-installable cartridge for the SpectraMax i3 and Paradigm Multi-Mode Platforms
Time-resolved fluorescence detection
Xenon flash lamp excitation source
- 340/80 nm excitation range
- 616/10 nm emission range
Supports nitrocellulose and PVDF membranes
Compatible with goat anti-rabbit, anti-mouse or streptavidin labeled antibodies
Reads membranes fitting the ScanLater SpectraMax Membrane Holder

Technologies of Western Blot Cartridge

Schematic representation of emission lifetimes that minimize background fluorescence

Schematic representation of emission lifetimes of background fluorescence and Eu-chelate showing principle of TRF measurement. Signal is measured after appropriate time-delay to reduce auto-fluorescence.

ScanLater Western Blot Assay Workflow
ScanLater Western Blot Assay Principle
Schematic of protein detection method shows the secondary antibody is directly labeled with Europium.
Europium-Chelate Excitation and Emission Spectra
Schematic representation of Europium-chelate excitation and emission spectra

Applikációk

Representative applications:

Protein identification and quantitation
Epitope mapping
Amino acid composition and sequence analysis
Spots imprinting
Phosphoprotein endogenous or exogenous expression
Protein resillience
Structure domain analysis
Protein expression
Protein content in serum
QC elimination of albumin and lgG in serum
Protein expression the cell cycle

Tartozékok, kiszerelések

Product Name	Description	Part Number
System ScanLater Western Blot Detection System	Membrane sample holder SoftMax Pro Software 6.3.1 Image plug-in for data analysis	0200-7027
Assay Kit ScanLater: Eu-Streptavidin Evaluation Kit	Europium-labeled streptavidin (20 μg) Blocking buffer (40 mL) Wash buffer (100 mL) For 10 mini gels with recommended protocol	R8200
Assay Kit ScanLater: Eu-Streptavidin Bulk Kit	Europium-labeled streptavidin (60 μg) Blocking buffer (120 mL) Wash buffer (300 mL) For 30 mini gels with recommended protocol	R8203
Assay Kit ScanLater: Anti-Mouse Evaluation Kit	Europium-labeled goat anti-mouse antibody (20 μg) Blocking buffer (40 mL) Wash buffer (100 mL) For 10 mini gels with recommended protocol	R8201
Assay Kit ScanLater: Anti-Mouse Bulk Kit	Europium-labeled goat anti-mouse antibody (60 μg) Blocking buffer (120 mL) Wash buffer (300 mL) For 30 mini gels with recommended protocol	R8205
Assay Kit ScanLater: Anti-Rabbit Evaluation Kit	Europium-labeled goat anti-rabbit antibody (20 μg) Blocking buffer (40 mL) Wash buffer (100 mL) For 10 mini gels with recommended protocol	R8202
Assay Kit ScanLater: Anti-Rabbit Bulk Kit	Europium-labeled goat anti-rabbit antibody (60 μg) Blocking buffer (120 mL), Wash buffer (300 mL) For 30 mini gels with recommended protocol	R8204
Assay Kit ScanLater: Anti-Goat Evaluation Kit	Europium-labeled donkey anti-goat antibody (20 μg) Blocking buffer (40 mL) Wash buffer (100 mL) For 10 mini gels with recommended protocol	R8224
Assay Kit ScanLater: Anti-Goat Bulk Kit	Europium-labeled donkey anti-goat antibody (60 μg) Blocking buffer (120 mL) Wash buffer (300 mL) For 30 mini gels with recommended protocol	R8227
Assay Kit ScanLater: Anti-Rat Evaluation Kit	Europium-labeled donkey anti-rat antibody (20 μg) Blocking buffer (40 mL) Wash buffer (100 mL) For 10 mini gels with recommended protocol	R8225
Assay Kit ScanLater: Anti-Rat Bulk Kit	Europium-labeled donkey anti-rat antibody (60 μg) Blocking buffer (120 mL) Wash buffer (300 mL) For 30 mini gels with recommended protocol	R8228
Protein Ladder ScanLater Western Blot Protein Ladder	7 biotinylated recombinant proteins at 10, 20, 40, 60, 80, 100, and 140 kD 3 pre-stained marker proteins at 18, 31, and 70 kD Ready to use, for 30 mini gels with recommended protocol	R8220
Assay Kit Components
ScanLater Wash Buffer (100 mL)		R8206
ScanLater 5x Blocking Buffer (120 mL)		R8207
ScanLater Goat Anti-Mouse Eu-labeled Antibody (60 µg)		R8208
ScanLater Goat Anti-Rabbit Eu-labeled Antibody (60 µg)		R8209
ScanLater Eu-labeled Antibody Streptavidin (60 µg)		R8212
ScanLater Eu-Anti-Goat IgG (60 μg)		R8226