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BIO SCIENCE
Molecular Devices

Comparison of Microplate Readers with Luminescence Detection


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 SpectraMax i3 Multi-Mode Detection PlatformSpectraMax Paradigm Multi-Mode Microplate ReaderSpectraMax M Series Multi-Mode Microplate ReadersFlexStation 3 Multi-Mode Microplate ReaderSpectraMax L Microplate Reader
Detector Photomultiplier tubes (PMT) and/or photodiode 2 PMT Photo-multiplier tube 2 PMT 1, 2, or 6 Ultra-fast Photomultiplier Tubes
Spectral range 300-850 nm 300-850 nm 250-850 nm 250-850 nm 380-630 nm
Dynamic range > 6 decades > 6 decades > 6 decades > 6 decades > 9 decades
Sensitivity 15 pM for 96-wells, 30 pM for 384-wells (Optimized for ATP-Glow) 1.25 pM for 96-wells, 3 pM for 384-wells, 19 pM for 1536-wells (Optimized for ATP-Glow) 43 pM ATP in 96 wells (optimized) < 20 attomol ATP per well
Microplate format 6 to 1536 wells* 6- to 1536-wells 6-384-well microplates 6-384 (96- and 384-wells with pipetting) 96- and 384-well microplates
Cross-talk < 0.3% in white 96- and 384-well microplates   <0.3% in white 96- and 384-well microplates <0.3% in white 96- and 384-well microplates < 3 x 10-5 (96-well)
Read modes Spectral, Endpoint, Kinetic Endpoint, Kinetic Endpoint, Kinetic Endpoint, Kinetic, Fast Kinetic Endpoint, Kinetic, Fast Kinetic, Dual-read
Read times 0:30 minutes for 96-wells, 1:15 minutes for 384-wells   2:00 minutes for 96-wells, 7:00 minutes for 384-wells 2:00 minutes for 96-wells, 7:00 minutes for 384-wells 0.1 up to 3600 sec. in steps of 0.1 sec.
Injectors/pipettors Option available Option available   8- or 16-channel pipettor 2 variable volume injectors
Injection/pipettor volumes       Max. volume 200uL for 8-channel and 30 uL for 16-channel  
Temperature control 4 degrees above ambient to 45 degrees C 2 degrees C above ambient to 45 degrees C 2 degrees C above ambient to 60 degrees C 2 degrees C above ambient to 45 degrees C 5 degrees C above ambient to 45 degrees C
Software SoftMax Pro Software SoftMax Pro Software SoftMax Pro Software SoftMax Pro Software SoftMax Pro Software
Validation & compliance tools IQ/OQ Protocols, SoftMax Pro GxP Compliance Software, SpectraTest LM1 Validation Plate SoftMax Pro GxP Compliance Software IQ/OQ Protocols, FDA 21 CFR Part 11 Compliance, SpectraTest LM1 Validation Plate IQ/OQ/PQ Protocols, FDA 21 CFR Part 11 Compliance Software Validation Package, IQ/OQ Documentation, Physical Validation Test Plate
Robotic compatible  x  x  x  x  x

* 1536 detection available via detection cartridges

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