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SALSA® digitalMLPA |
SALSA® digitalMLPA is similar to conventional MLPA, the gold standard for CNV detection of multiple DNA sequences, but with the ability to examine many more targets. digitalMLPA takes the guesswork out of NGS CNV calls by combining the MLPA technology you trust for CNV detection with the large number of targets you have come to expect with NGS. With digitalMLPA, you can examine more targets than with conventional MLPA, with decreased costs and turnaround times.
ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK!
SALSA® digitalMLPA – where MLPA meets NGS
The fastest way to broad copy number certainty
✓ Reliable: proven copy number detection technology+
✓ Robust: only 20 ng of sample DNA needed; uniform coverage
✓ Quality control: control probes included in each reaction
✓ Simple: easy hands-on steps and no library quantification needed
✓ Cost-effective: up to 1000 probes in one reaction
✓ Sample certainty: SNV probes present to distinguish samples
✓ Easy analysis: user-friendly analysis with free Coffalyser digitalMLPA software
SALSA® digital Multiplex Ligation-dependent Probe Amplification (digitalMLPA) is a
multiplex PCR followed by Illumina sequencing-based amplicon quantification, for the
detection of copy number variations (CNV) and specific point mutations. digitalMLPA
amplifies ligated probes with a universal primer pair enabling unbiased amplification.
With digitalMLPA, up to 1000 unique sequences can be detected and quantified in a
single reaction.
FIRST digitalMLPA ASSAY ON THE MARKET in 2020:
SALSA digitalMLPA Probemix D001 Hereditary Cancer Panel 1
digitalMLPA products are for research use only (RUO).
Videók, ismertetők
SALSA® digitalMLPA Confidence in Copy Number Determination termékismertető letöltése (PDF)
SALSA® digitalMLPA General Protocol (pdf) - for use with Illumina sequencing platforms
digitalMLPA Protocol Supplements (pdf) - Running digitalMLPA reactions on NextSeq 500/550 instruments
and Combining digitalMLPA reactions and standard paired-end sequencing reactions. For detailed informations please visit https://www.mrcholland.com/technology/digitalmlpa
What is digitalMLPA?
digitalMLPA is a technique for the copy number quantification of up to 1000 target sequences. The technique combines the conventional MLPA technology with next-generation sequencing (NGS). This video briefly describes what digitalMLPA is and what steps are involved.
In case you're unable to view the video on YouTube you can also download it here.
Applikációk
SALSA digitalMLPA Probemix D001 Hereditary Cancer Panel 1
The digitalMLPA probemix D001 detects CNVs in 28 clinically relevant genes, and detects six specific common (point) mutations. Mutations in these genes are associated with hereditary predisposition to one or more of the following cancer types: breast, ovarian, colorectal, gastric, prostate, pancreatic, endometrial, and melanoma. Read more about the targeted genes on the product page.
Tartozékok, kiszerelések
Coffalyser digitalMLPA
Coffalyser digitalMLPA is free software developed by MRC Holland for the analysis of digitalMLPA data. Coffalyser digitalMLPA automatically recognises digitalMLPA sequence reads from FASTQ files and directly uses them for analysis. Coffalyser digitalMLPA then outputs two clear reports for every sample of your experiment indicating both the quality of the run and the aberrations found.
https://www.mrcholland.com/technology/software/coffalyser-digitalmlpa
Coffalyser digitalMLPA is for research use only (RUO).
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